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Documenti fondamentali

MABC1607M

Sigma-Aldrich

Anti-MMP-11 (Stromelysin-3) Antibody, clone 5ST-4A9

Sinonimo/i:

Stromelysin-3, SL-3, ST3, MMP11, Matrixmetalloproteinase 11

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

monoclonal

Reattività contro le specie

human

tecniche

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1λ

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

ambient

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Stromelysin-3 (UniProt: P24347; also known as SL-3, ST3, Matrix metalloproteinase-11, MMP-11) is encoded by the MMP11 (also known as STMY3) gene (Gene ID: 4320) in human. MMP-11 is shown to be differentially expressed in tumor tissue and plays a role in tumor invasion. It is shown to be well expressed in stromal cells of breast carcinomas. It has little expression in adult organs under normal physiological conditions. During development, it is expressed in many processes involved in cell migration and tissue morphogenesis. MMP-11 has multiple zinc and calcium binding sites. It binds 2 zinc ions per subunit and one calcium ion per subunit. MMP-11 contains 4 hemopexin domains (aa 291-339; 340-382; 384-432; and 433-480). It is synthesized with a signal peptide (aa 1-31) and a propeptide region (aa 32-97) that are cleaved to generate mature enzyme. It contains a conserved cysteine that is present in the cysteine-switch motif (aa 78-85) that binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion activates the enzyme. The precursor is cleaved by a furin endopeptidase. Expression levels of MMP-11 are a reliable indicator of greatest risk for cancer recurrence in breast carcinoma, pancreatic tumors, and colon cancer. (Ref.: Roscilli, G et al. (2014). J. Transl. Med. 12; 54).

Specificità

Clone 5ST-4A9 detects MMP-11 (stromelysin 3) in human cells.

Immunogeno

His-tagged full length recombinant human MMP-11 (Stromelysin-3).

Applicazioni

Immunohistochemistry Analysis: A representative lot detected MMP-11 (Stromelysin-3) in Immunohistochemistry applications in (Ahmad, A., et. al. (1998) Am J Pathol. 152(3):721-8; Nakopoulou, L., et. al. (2002). Mod Pathol. 15(11):1154-61).

Western Blotting Analysis: A representative lot detected MMP-11 (Stromelysin-3) in Western Blotting applications in (von Marschall, Z., et. al. (1998) Gut. 43(5):692-8; Motrescu, E.R., et. al. (2008). Oncogene. 27(49):6347-55; Luo, D., et. al. (1999). J Biol Chem. 274(52):37177-85).

Immunohistochemistry Analysis: A representative lot detected MMP-11 (Stromelysin-3) in Immunohistochemistry applications in (Cribier, B., et. al. (2002). J Am Acad Dermatol. 46(3):408-13; von Marschall, Z., et. al. (1998) Gut. 43(5):692-8; Andarawewa, K.L., et. al. (2005). Cancer Res. 65(23):10862-71; Motrescu, E.R., et. al. (2008). Oncogene. 27(49):6347-55.

Immunoprecipitation Analysis: A representative lot detected MMP-11 (Stromelysin-3) in Immunoprecipitation applications in (Schonbeck, U., et. al. (1999). J Exp Med. 189(5):843-53).

Immunohistochemistry Analysis: A 1:50, 250, 1,000 dilution from a representative detected MMP-11 (Stromelysin-3) in human placenta and human breast cancer tissue.
Research Category
Apoptosis & Cancer

Qualità

Evaluated by Western Blotting in PC-3 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected MMP-11 (Stromelysin-3) in 10 µg of PC-3 cell lysate.

Descrizione del bersaglio

~55 kDa observed; 54.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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