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Documenti fondamentali

MAB8879

Sigma-Aldrich

Anti-Cdk4 Antibody, clone DCS-35

clone DCS-35, Chemicon®, from mouse

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

DCS-35, monoclonal

Reattività contro le specie

mouse, pig, rat, human

Produttore/marchio commerciale

Chemicon®

tecniche

activity assay: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1κ

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... CDK4(1019)
rat ... Cdk4(94201)

Descrizione generale

The prototype of the cdk family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.

Specificità

Recognizes a 34 kDa protein identified as p34 or cyclin-dependent kinase 4 (cdk4). It shows no cross-reaction with other cdks.

Immunogeno

Raised against purified recombinant cdk4 protein.

Applicazioni

Detect Cdk4 with Anti-Cdk4 Antibody, clone DCS-35 (Mouse Monoclonal Antibody), that has been shown to work in IF, IP, EA, WB & IHC.
Immunofluorescence

Immunoprecipitation: excellent for coprecipitation of D-type cyclins bound to cdk4.

Western Blotting: 2-4 ug/mL

Kinase Assay

The prototype of the cdk family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Linkage

Replaces: MABE548

Stato fisico

Format: Purified
Purified immunoglobulin. Liquid containing 10 mM PBS, pH 7.4 with 0.2% BSA and 0.09% sodium azide.

Stoccaggio e stabilità

Maintain liquid material at 2-8°C for up to 24 months.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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The immunohistochemical profile of the primary and metastatic carcinoma of the clitoris: a case report.
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Chinese Journal of Integrative Medicine null
Cell cycle regulators cyclin D1 and CDK4/6 have estrogen receptor-dependent divergent functions in breast cancer migration and stem cell-like activity.
Lamb, R; Lehn, S; Rogerson, L; Clarke, RB; Landberg, G
Cell Cycle null
Effects of quercetin on CDK4 mRNA and protein expression in A549 cells infected by H1N1.
Wan, Q; Wang, H; Lin, Y; Gu, L; Han, M; Yang, Z; Zhang, Y; Ma, R; Wang, L; Wang, Z
Biomedical Reports null
Expression of the cell-cycle regulatory proteins (pRb, cyclin D1, p16INK4A and cdk4) in human endometrial cancer: correlation with clinicopathological features.
Andrzej Semczuk, Roman Miturski, Danuta Skomra, Jerzy A Jakowicki
Archives of Gynecology and Obstetrics null

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