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MAB4360C3

Sigma-Aldrich

Anti-TRA-1-60 Antibody, clone TRA-1-60, Cy3 conjugate

clone TRA-1-60, from mouse, CY3 conjugate

Sinonimo/i:

TRA160, Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Coniugato

CY3 conjugate

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

TRA-1-60, monoclonal

Reattività contro le specie

human

tecniche

immunocytochemistry: suitable

Isotipo

IgM

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... PODXL(5420)

Descrizione generale

TRA-1-60 is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA-1-60 reacts with a neuraminidase sensitive epitope of a high molecular weight glycoprotein that are down-regulated upon differentiation. Recently this antigen has been proposed to be a form of the protein podocalyxin. TRA-1-60, can be detected in the serum of germ cell tumor patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.

Specificità

This antibody recognizes human TRA-1-60.

Immunogeno

Human embryonal carcinoma cell line 2102Ep.

Applicazioni

Anti-TRA-1-60 Antibody, clone TRA-1-60, Cy3 conjugate is an antibody against TRA-1-60 for use in ICC.
Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody did not detect TRA-1-60 in mouse embryonic stem cells (SCR012).
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Qualità

Evaluated by Immunocytochemistry in H9 human embryonic stem cells.
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected TRA-1-60 in H9 human embryonic stem cells.

Descrizione del bersaglio

235/410 kDa calculated

Stato fisico

Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Stoccaggio e stabilità

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Risultati analitici

Control
H9 human embryonic stem cells

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Human embryonic stem cells reprogram myeloid precursors following cell-cell fusion.
Yu, Junying, et al.
Stem Cells, 24, 168-176 (2006)
Direct conversion of human fibroblasts to multilineage blood progenitors.
Szabo, Eva, et al.
Nature, 468, 521-526 (2010)
Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells.
Andrews, P W, et al.
Hybridoma, 3, 347-361 (1984)
Athurva Gore et al.
Nature, 471(7336), 63-67 (2011-03-04)
Defined transcription factors can induce epigenetic reprogramming of adult mammalian cells into induced pluripotent stem cells. Although DNA factors are integrated during some reprogramming methods, it is unknown whether the genome remains unchanged at the single nucleotide level. Here we
Daniel Rodrigo Marinowic et al.
Molecular medicine reports, 15(4), 2049-2056 (2017-03-06)
Focal cortical dysplasia (FCD) is caused by numerous alterations, which can be divided into abnormalities of the cortical architecture and cytological variations; however, the exact etiology of FCD remains unknown. The generation of induced pluripotent stem cells (iPSCs) from the

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