MAB3300

Anti-TIMP-1 Antibody, clone 7-6C1

clone 7-6C1, Chemicon^®, from mouse

• Sinonimo/i: Tissue Inhibitor of Metalloproteinase-1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 7-6C1, monoclonal
• Reattività contro le specie: rat, human, bovine
• Non deve reagire con: mouse
• Produttore/marchio commerciale: Chemicon^®
• tecniche: ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NM_003254.2
• N° accesso UniProt: P01033
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TIMP1(7076)

Descrizione

Descrizione generale

Tissue Inhibitors of Metalloproteinases (TIMPs) inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-1 and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kDa type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-1 levels in quiescent cells and tissues is low (pg/ml), and stimulation with the phorbol ester TPA or protein concentration is often needed to visualize protein bands. In addition, cell types differ greatly in the amount of TIMP-1 produced.

Specificità

MAB3300 is a purified mouse monoclonal antibody to bovine tissue inhibitor of metalloproteinases-1 (bovine TIMP-1). The antibody specifically reacts with bovine TIMP-1 and cross-reacts with human and rat TIMP-1, but not with mouse TIMP-1. By western blot the antibody recognizes a glycoprotein of 28.5 kDa, which is identified as tissue inhibitor of metalloproteinases-1 (TIMP-1).

Immunogeno

Bovine TIMP-1

Applicazioni

Detect TIMP-1 using this Anti-TIMP-1 Antibody, clone 7-6C1 validated for use in ELISA, WB, IH.

Immunoblotting: 1:1000, identifies a band of approximately 30KDa. Note TIMP-1 is found in very low concentrations in most cells and tissues.Positive control is HT1080 cells treated with PMA or recombinant TIMP-1 protein CC3328.

Immunohistochemistry on frozen sections or acetone fixed tissues. Formalin fixed paraffin-embedded tissue sections are usually not reactive. PLP fixation is suggested as an alternative.

EIA

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Linkage

Replaces: 04-1133

Stato fisico

Format: Purified

Purified immunoglobulin presented as a liquid in 0.1 M Na-Phosphate buffer, pH 7.0 containing protease free bovine serum albumin.

Stoccaggio e stabilità

Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable