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MAB16990

Sigma-Aldrich

Anti-Hepatitis B Virus Antibody, core Antigen (ayw), clone 14E11, a.a. 134-140

ascites fluid, clone 14E11, Chemicon®

Sinonimo/i:

HBcAg

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

primary antibodies

Clone

14E11, monoclonal

Reattività contro le specie

human

Produttore/marchio commerciale

Chemicon®

tecniche

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2b

Condizioni di spedizione

wet ice

Specificità

Reacts with HBcAg (epitope recognized - amino acid positions 135-140).

SPECIES REACTIVITIES:

Cross-reacts with woodchuck hepatitis virus core antigen (WHcAg), but not with duck hepatitis B virus core antigen (DHBcAg).

Immunogeno

Epitope: Core Antigen (ayw)
Recombinant purified HBcAg from E.coli, SDS-denatured

Applicazioni

ELISA, immunoblotting, immunofluorescent and immunoperoxidase staining of infected liver tissues and transfected cultured cells.

Recommended working dilutions: for immunoblotting - 1:1000; for immunofluorescence - 1:200. Optimal working dilutions must be determined by the end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
This Anti-Hepatitis B Virus Antibody, core antigen (ayw), clone 14E11, a.a. 134-140 is validated for use in ELISA, IF, IP, WB, IC, IH for the detection of Hepatitis B Virus.

Stato fisico

Ascites. Liquid, containing 0.1% sodium azide.

Stoccaggio e stabilità

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Boerries Brandenburg et al.
Hepatology (Baltimore, Md.), 42(6), 1300-1309 (2005-12-01)
Protein transduction domains (PTDs) have been used to deliver a variety of biologically active cargo across cellular membranes. However the potential of PTDs to mediate transport of nanoparticular structures into the cytoplasm bypassing the endosomal compartment remains unclear. Cell-permeable virus-like
Immunochemical structure of the carboxy-terminal part of hepatitis B e antigen: identification of internal and surface-exposed sequences
Sallberg, M, et al
The Journal of General Virology, 74 ( Pt 7), 1335-1340 (1993)
The structure of the variable regions of mouse monoclonal antibodies to hepatitis B virus core antigen.
Skrivelis, V, et al.
Scandinavian Journal of Immunology, 37, 637-643 (1993)
V Bichko et al.
Molecular immunology, 30(3), 221-231 (1993-02-01)
Particulate and denatured core protein as well as e-antigen (HBe) of hepatitis B virus (HBV) differ in part immunologically but this has not been studied in sufficient detail. Therefore, in this study the B-cell immune response to native and denatured
The affinities of monoclonal antibodies against core antigen of hepatitis B virus.
Apsalons, U and Bichko, V
Archives of Virology, 134, 393-402 (1994)

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