MAB13444

Anti-Integrin α6 Antibody, clone BnC6B4

clone BnC6B4, Chemicon^®, from mouse

• Sinonimo/i: CD49f
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: BnC6B4, monoclonal
• Reattività contro le specie: chicken
• Produttore/marchio commerciale: Chemicon^®
• tecniche: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1
• N° accesso NCBI: NM_000210.2; NM_001079818.1
• N° accesso UniProt: P23229
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ITGA6(3655)

Descrizione

Specificità

Reactive with chicken integrin alpha6, as demonstrated by experiments with cDNA-transfected NIH3T3 cells followed by immunoblots.

Immunogeno

Integrin alpha subunits purified from adult chicken brain tissue, denatured in 6M guanidine isothiocyanate, and depleted of beta1 subunit.

Applicazioni

Anti-Integrin α6 Antibody, clone BnC6B4 is an antibody against Integrin α6 for use in IP, WB, IC, IH.

Western blot

Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor.

Immunohistochemistry: Effective for immunohistochemistry of methanol-fixed cryosections. An overnight methanol fix is suggested. Previous experiments have used 14 micron sections of chicken embryonic tissue. Typical antibodies dilutions are 35 μg/mL, with overnight antibody incubation, followed by detection with a goat anti-mouse Ig -FITC secondary antiobody.

Immunocytochemistry: Effective for methanol-fixed cultured cells (15 minute fixation).

Immunoblot: Recognizes a 130 kDa band in chicken brain tissue and a 140 kDa band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation).

Optimal working dilutions must be determined by end user.

Stato fisico

Format: Purified

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable