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Key Documents

MAB1273B

Sigma-Aldrich

Anti-Mitochondria Antibody, clone 113-1, Biotin Conjugate

clone 113-1, from mouse, biotin conjugate

Sinonimo/i:

Human Mitochondria

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Coniugato

biotin conjugate

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

113-1, monoclonal

Reattività contro le specie

human (mitochondria protein)

Non deve reagire con

rat (mitochondria protein), mouse (mitochondria protein)

tecniche

immunocytochemistry: suitable
immunohistochemistry: suitable

Isotipo

IgG1

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions. MAB1273B recognizes a 65 kD protein by immunoprecipitation and produces the stringy, "spaghetti-like", staining patten in the cytoplasm of human cells.

Specificità

This antibody recognizes human. Antibody is not reactive with mouse or rodent.

Immunogeno

Surface of intact human mitochondria

Applicazioni

Detect Mitochondrial Marker using this mouse monoclonal antibody, Anti-Mitochondria Antibody, clone 113-1, Biotin Conjugate validated for use in ICC & IHC.
Evaluated by Immunocytochemistry in primary mouse embryonic fibroblasts (PMEFs).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody did not detect mitrochondria in primary mouse embryonic fibroblasts (PMEFs). Evaluated by Immunohistochemistry in human kidney tissue.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected mitrochondria in human kidney tissue.
Research Category
Stem Cell Research

Cell Structure
Research Sub Category
Developmental Neuroscience

Organelle & Cell Markers

Qualità

Evaluated by Immunocytochemistry in human adipose mesenchymal stem cells (SCC038).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected mitrochondria in human adipose mesenchymal stem cells (SCC038).

Descrizione del bersaglio

60 kDa calculated

Stato fisico

Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.

Stoccaggio e stabilità

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Risultati analitici

Control
Human adipose mesenchymal stem cells (SCC038).

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Emily Pulford et al.
Pathology, 48(7), 650-659 (2016-12-14)
Vasculogenic mimicry, the process in which cancer cells form angiomatoid structures independent of or in addition to host angiogenesis has been recorded in several otherwise non-endothelial malignant neoplasms. This study describes evidence of routine vascular mimicry by human mesothelioma cell
Kai Kang et al.
Biomaterials, 230, 119574-119574 (2019-11-26)
Engineered heart tissues (EHTs) are regarded as being the most promising alternative to synthetic materials, and autologous mesenchymal stem cells (MSCs) are widely used as seeding cells. However, few studies have evaluated the feasibility of using MSCs from patients with

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