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CBL154

Sigma-Aldrich

Anti-H-CAM Antibody, clone F10-44-2

clone F10-44-2, Chemicon®, from mouse

Sinonimo/i:

CD44

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

100

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

F10-44-2, monoclonal

Reattività contro le specie

human

Produttore/marchio commerciale

Chemicon®

tecniche

flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

Isotipo

IgG2a

N° accesso NCBI

NM_000610.3
NM_001001389.1
NM_001001390.1
NM_001001391.1
NM_001001392.1

N° accesso UniProt

P16070

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... CD44(960)

Categorie correlate

Applicazioni

Identification of CD44 positive cells by flow cytometry and immunocytochemistry, in particular with formalin fixed paraffin wax embedded sections of lymphoid and epithelial tissues (high temperature antigen retrieval in 10mM citrate buffer, pH6.0, is recommended).

Studies involving T, B cell, monocyte, granulocyte T and B cell binding to HEV during lymphocyte circulation and movement of leucocytes to inflammatory sites.

Studies on HCAM (homing receptor) function.

Immunoprecipitation.

Optimal working dilutions must be determined by the end user.
This Anti-H-CAM Antibody, clone F10-44-2 is validated for use in FC, IP, IH, IH(P) for the detection of H-CAM.

Stato fisico

Format: Purified

Risultati analitici

Control
POSITIVE CONTROL: Tonsil; stains all T-cells in the paracortex

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Biochemical characterisation and localization in brain of a human brain-leucocyte membrane glycoprotein recognised by a monoclonal antibody.
McKenzie, J L, et al.
Journal of Neurochemistry, 39, 1461-1466 (1982)
Identification of mRNA that encodes an alternative form of H-CAM(CD44) in lymphoid and nonlymphoid tissues.
Goldstein, L A and Butcher, E C
Immunogenetics, 32, 389-397 (1990)
José M Díez et al.
Stem cell research & therapy, 6, 28-28 (2015-04-19)
Fetal bovine serum (FBS) is an animal product used as a medium supplement. The animal origin of FBS is a concern if cultured stem cells are to be utilized for human cell therapy. Therefore, a substitute for FBS is desirable.
Arantxa Blázquez-Prunera et al.
Stem cell research & therapy, 8(1), 103-103 (2017-04-30)
Mesenchymal stem cells (MSCs) show promising characteristics for their use in advanced therapy medicinal products. However, there are some unresolved concerns, such as the use of animal components for their expansion. In this study we assessed the suitability of a

Articoli

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Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Protocolli

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

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