ABN37
Anti-Homer1 Antibody
from rabbit, purified by affinity chromatography
Sinonimo/i:
Homer protein homolog 1, Homer-1, Homer1
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Purificato mediante
affinity chromatography
Reattività contro le specie
human, mouse
Reattività contro le specie (prevista in base all’omologia)
horse (based on 100% sequence homology), monkey (based on 100% sequence homology)
tecniche
immunohistochemistry: suitable (paraffin)
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... HOMER1(9456)
Descrizione generale
Homer protein homolog 1 (UniProt Q86YM7; also known as Homer-1, Homer1) is encoded by the HOMER1 (also known as SYN47) gene (Gene ID 9456) in human. Homer-1, -2, and -3 constitute a family of postsynaptic scaffold proteins that play a central role in calcium signaling. Homer members share a conserved N-terminal Ena/VASP homology 1 (EVH1) domain (a.a. 1-110 of Homer-1) that mediate signaling complexes assembly by interacting with PPXXFR proline-rich consensus sequences found in various other scaffolding and signal transduction molecules, including metabotropic glutamate receptors (mGluRs) and 1,4,5-trisphosphate receptors (IP3Rs). In addition to mediate group I mGluRs signaling via their EVH1 domain, Homer-1 spliced variants Vesl-1L, 1b, and 1d, but not Vesl-1M or Vesl-1S, also contain a C-terminal coiled-coil (a.a. 181-352) region that mediate self-multimerization. Homer-1 variants are associated with the etiology of many neurological diseases, such as chronic pain, mental retardation syndromes, Alzheimer′s disease (AD), Parkinson′s disease (PD), schizophrenia, drug-induced addiction, and traumatic brain injury (TBI). Homer1b/c-knockdown is reported to protect against chemically induced seizure in mice as a result of preventing mGluR5-mediated mTOR pathway activation.
Specificità
Broad species reactivity expected based on high sequence homology.
This rabbit polyclonal antibody targets an internal sequence within the N-terminal half of human Homer1 isoform 1b and mouse isoform Vesl-1L. Immunogen sequence appears near the C-terminus of human isoform 1h and mouse isoforms 1b, 1d, Vesl-1M, and Vesl-1S, but absent in human isoform 1e. Highly homologous sequence (~83%) is also found in the N-terminal half of rat isoform 1c (Vesl-1L) and near the C-terminus of rat isoforms 1a (Vesl) and 1b.
Immunogeno
KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human Homer1 isoform 1b and near the C-terminus of isoform 1h.
Applicazioni
Anti-Homer1, Cat. No. ABN37, is a highly specific rabbit polyclonal antibody that targets Homer protein homolog 1 and has been tested in Immunohistochemistry and Western Blotting.
Immunohsitochemistry Analysis: A 1:500 dilution from a representative lot detected Homer1 in human and mouse cerebral cortex tissue sections.
Immunohsitochemistry Analysis: A representative lot detected significantly increased stratum radiatum and stratum oriens Homer1 immunoreactivity in floating brain sections from mice received AP-12 intraperitoneal injection (Jansone, B., et al. (2015). PLoS One. 10(6):e0127686).
Immunohsitochemistry Analysis: A representative lot detected significantly increased stratum radiatum and stratum oriens Homer1 immunoreactivity in floating brain sections from mice received AP-12 intraperitoneal injection (Jansone, B., et al. (2015). PLoS One. 10(6):e0127686).
Qualità
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Homer1 in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Homer1 in 10 µg of mouse brain tissue lysate.
Descrizione del bersaglio
~47 kDa observed. 40.28/19.86 kDa (human isoform 1b/1h), 40.22/42.08/41.41/22.78/20.91 kDa (mouse isoform 1b/1d/Vesl-1L/Vesl-1M/Vesl-1S) calculated. Uncharacterized bands may be observed in some lysate(s).
Altre note
Concentration: Please refer to lot specific datasheet.
Non trovi il prodotto giusto?
Prova il nostro Motore di ricerca dei prodotti.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Jie Wang et al.
Cell, 184(24), 5869-5885 (2021-11-11)
RTN4-binding proteins were widely studied as "NoGo" receptors, but their physiological interactors and roles remain elusive. Similarly, BAI adhesion-GPCRs were associated with numerous activities, but their ligands and functions remain unclear. Using unbiased approaches, we observed an unexpected convergence: RTN4
A Novel 1,4-Dihydropyridine Derivative Improves Spatial Learning and Memory and Modifies Brain Protein Expression in Wild Type and Transgenic APPSweDI Mice.
Jansone, B; Kadish, I; van Groen, T; Beitnere, U; Moore, DR; Plotniece, A; Pajuste, K; Klusa, V
Testing null
Myung-Su Kang et al.
The Journal of experimental medicine, 214(10), 2947-2966 (2017-09-14)
Vaccinia-related kinases (VRKs) are multifaceted serine/threonine kinases that play essential roles in various aspects of cell signaling, cell cycle progression, apoptosis, and neuronal development and differentiation. However, the neuronal function of VRK3 is still unknown despite its etiological potential in
Hongwon Kim et al.
Frontiers in aging neuroscience, 13, 709091-709091 (2021-08-24)
Recent evidence indicates that gut microbiota could interact with the central nervous system and affect brain function, including cognition and memory. In this study, we investigated whether Bifidobacterium bifidum BGN4 (B. bifidum BGN4) and Bifidobacterium longum BORI (B. longum BORI)
Fujun Luo et al.
Nature communications, 12(1), 2380-2380 (2021-04-24)
Diverse signaling complexes are precisely assembled at the presynaptic active zone for dynamic modulation of synaptic transmission and synaptic plasticity. Presynaptic GABAB-receptors nucleate critical signaling complexes regulating neurotransmitter release at most synapses. However, the molecular mechanisms underlying assembly of GABAB-receptor
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.