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Key Documents

AB5471P

Sigma-Aldrich

Anti-Semaphorin 3F Antibody

Chemicon®, from rabbit

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

affinity purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

rat, human

Produttore/marchio commerciale

Chemicon®

tecniche

immunocytochemistry: suitable
western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... SEMA3F(6405)

Specificità

Recognizes Semaphorin 3F (Sema3F), recombinant and purified protein. By Western blot the antibody recognizes a protein of 90 kDa in total extracts from tissue culture cells producing Sema3F. Semaphorin 3F is expressed ubiquitously with higher levels in lung, brain, spleen, muscle and heart.
This antibody may also react with mouse because of extremely high sequence conservation in the SEM3F protein.

Immunogeno

Synthetic peptide from human Semaphorin 3F (Catalog number AG363). (TPWTQTQAVRFRFSRATDGALRPM).

Applicazioni

Anti-Semaphorin 3F Antibody is an antibody against Semaphorin 3F for use in IC & WB.
Research Category
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
Western blot: 0.5-1.0 μg/mL using ECL. Suggested antibody dilution buffer is TBST (10 mM Tris-HCl, pH 8.0, 150 mM NaCl with 0.05% Tween 20). Suggested incubation is for two hours at room temperature or overnight at 2-8°C.
Immunocytochemistry.

Optimal working dilutions must be determined by the end user.

Descrizione del bersaglio

90 kDa

Linkage

Replaces: AB5471

Stato fisico

Affinity Purified immunoglobulin. Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
ImmunoAffinity Purified

Stoccaggio e stabilità

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Risultati analitici

Control
Embryonic brain tissue, neural growth cones

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Endogenous axon guiding chemorepulsant semaphorin-3F inhibits the growth and metastasis of colorectal carcinoma.
Wu, F; Zhou, Q; Yang, J; Duan, GJ; Ou, JJ; Zhang, R; Pan, F; Peng, QP; Tan, H; Ping et al.
Clinical cancer research : an official journal of the American Association for Cancer Research null
Expression of class 3 semaphorins and their receptors in human breast neoplasia.
Carolyn A Staton,Lucy A Shaw,Manoj Valluru,Leslie Hoh,Ivan Koay,Simon S Cross et al.
Histopathology null
Identification of genes differentially expressed in rat alveolar type I cells
Dahlin, Katherine, et al.
American Journal of Respiratory Cell and Molecular Biology, 31, 309-316 (2004)
Alexander Fassold et al.
Arthritis and rheumatism, 60(10), 2892-2901 (2009-10-01)
In inflammatory lesions, sympathetic nerve fibers disappear soon after the start of inflammation. We identified sympathetic nerve repellents as possible causal agents in rheumatoid arthritis (RA). On nerve terminals, repellent factors bind to neuropilin-2 and its coreceptor. The aim of
Benoit Favier et al.
Blood, 108(4), 1243-1250 (2006-04-20)
Neuropilin 2 (NRP2) is a receptor for the vascular endothelial growth factor (VEGF) and the semaphorin (SEMA) families, 2 unrelated ligand families involved in angiogenesis and neuronal guidance. NRP2 specifically binds VEGF-A and VEGF-C, although the biological relevance of these

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