AB5458

Anti-Neprilysin Antibody (Neutral Endopeptidase, Nep)

serum, Chemicon^®

• Sinonimo/i: neprilysin, Neprilysin, Enkephalinase, enkephalinase, atriopeptidase, Atriopeptidase, Neutral Endopeptidase, NEP, Endopeptidase 24.11, CD10
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: serum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: rat, mouse, human
• Produttore/marchio commerciale: Chemicon^®
• tecniche: immunohistochemistry: suitable; western blot: suitable
• N° accesso NCBI: NM_000902.3
• N° accesso UniProt: P08473
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... MME(4311)

Descrizione

Descrizione generale

Human neutral endopeptidase (Neprilysin) is single pass type II integral membrane endopeptidase that functions to activate and degrade biologically active enzymes. Endopeptidase,in general, function to cleave peptide bonds within enzyme sequences, in contrast to exopeptidases that target the end sequence. Neprilysin is part of the peptidase M13 family that acts on polypeptides of less than 30 amino acids with Thermolysin-like specificity for cleave sites. Thermolysin is thought to cleave before amino acids Ile, Met, Phe, Trpm or Val (unless Pro precedes) and after Ala, Asp, His, or Thr. Neprilysin preferentially cleaves polypeptides between hydrophobic residues, particularly with Phe or Tyr at position 1. Neprilysin research has found it important in the degradation of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Research has found it may function in the degradation of atrial natriuretic factor (ANF). Upregulation of ANF functions to regulate homeostatis of water, sodium, potassium and adipose in response to high blood pressure in muscle cells of the heart.

Specificità

Reactivity with other species has not been confirmed.

Recognizes Neprilysin (Neutral Endopeptidase, NEP). On western blots of rat kidney extracts the antibody detects a band migrating at approximately 86 kDa corresponding to neprilysin.

Immunogeno

Rat Neprilysin.

Applicazioni

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This Anti-Neprilysin Antibody (Neutral Endopeptidase, Nep) is validated for use in IH, WB for the detection of Neprilysin (Neutral Endopeptidase).

Western Blotting:
A previous lot of this antibody was used on Western Blotting.

Immunohistochemistry:
A previous lot of this antibody was used on Immunohistochemistry.

Optimal working dilutions must be determined by the end user.

Descrizione del bersaglio

~86 kDa

Stato fisico

Rabbit polyclonal serum. Contains no preservative.

Unpurified

Stoccaggio e stabilità

Stable for 1 year at -20ºC from date of receipt.

Risultati analitici

Control
Kidney tissue.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable