69066-M

Recombinant Enterokinase

• Sinonimo/i: Enterokinase Enzyme, Enterokinase Protein, Recombinant Enterokinase
• Codice UNSPSC: 12352202
• NACRES: NA.54

Proprietà

• Origine biologica: bovine
• Livello qualitativo: 200
• Ricombinante: expressed in E. coli
• Stato: liquid
• Produttore/marchio commerciale: Novagen^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• tecniche: targeted protein degradation: suitable
• Compatibilità: suitable for enzyme test
• applicazioni: diagnostic assay manufacturing
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Recombinant Enterokinase (rEK) is a highly purified preparation of the catalytic subunit of bovine enterokinase, a serine protease which recognizes the identical cleavage site as the native enzyme (AspAspAspAspLys↓) and has similar enzymatic activity. This enzyme is produced in E. coli and purified to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC 3.4.21.9, M.W. 26,300.

Componenti

•50 URecombinant Enterokinase

•2 ml1X rEK Dilution/Storage Buffer

•1 ml10X rEK Cleavage Buffer

•10 µgCleavage Control Protein

Attenzione

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Definizione di unità

One unit is defined as the amount of enzyme that will cleave 50 µg of fusion protein in 16 hours at 23°C, in a buffer containing 20 mM Tris-HCl pH 7.4, 50 mM NaCl, 2 mM CaCl₂.

Altre note

Collins-Racie, L.A. et al. 1995. Bio/Technology 13, 982.

Note legali

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable