69670-M

His•Bind^® Resin

• Sinonimo/i: uncharged IDA agarose beads
• Codice UNSPSC: 41106500
• NACRES: NA.56

Proprietà

• Forma fisica: liquid
• Produttore/marchio commerciale: Novagen^®
• Condizioni di stoccaggio: do not freeze
• applicazioni: life science and biopharma
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

His•Bind Resin is an uncharged IDA agarose resin. The binding capacity is approximately 8 mg/ml bed volume. His•Bind Resin is well suited to small to medium scale purifications using either gravity flow columns or batch methods.

Applicazioni

His•Bind^® Resin have been used: in purification of His•Tag^® fusion proteins by metal chelation chromatography, for purification of the bacterially produced OCRL1WT/VAR phosphatase domain to study heterogeneity in Lowe Syndromein protein purification to study anaerobic phloroglucinol degradation by Clostridium scatologenes for isolation of His6-tagged proteins for studying the role of human repressor activator protein 1 (RAP1) and glial fibrillary acidic protein (GFAPɛ)proteins in increasing γ-secretase activity in a yeast model system.

Azioni biochim/fisiol

His•Bind^® resin helps in the purification of proteins containing the His-tagged sequence by the process of metal chelation chromatography. It utilizes the charge buffer provided in the His•Bind^® Buffer Kit, that binds the His-tagged sequence effectively with Ni²⁺ or Co²⁺ cations that are immobilized onto the His•Bind^® resin. The target protein can be easily recovered by eluting with imidazole. The His•Bind^® Resin is designed to be regenerated and reused multiple times, making it a cost-effective solution. Its versatility allows for protein purification under gentle, non-denaturing conditions, or in the presence of either guanidine or urea, depending on the specific requirements of the purification process.

Caratteristiche e vantaggi

• Reusable many times
• Compatible with His•Bind^® Buffer Kit
• Compatible with 1 mM THP

Attenzione

Toxicity: Standard Handling (A)

Note legali

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS02
• Avvertenze: Warning
• Indicazioni di pericolo: H226
• Consigli di prudenza: P210 - P233 - P240 - P241 - P242 - P243
• Classi di pericolo: Flam. Liq. 3
• Codice della classe di stoccaggio: 3 - Flammable liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): 86.0 °F
• Punto d’infiammabilità (°C): 30 °C