616464

SB431542

≥97% (HPLC), liquid, TGF-β RI kinase inhibitor, Calbiochem^®

• Sinonimo/i: InSolution TGF-β RI Kinase Inhibitor VI, SB431542
• Formula empirica (notazione di Hill): C₂₂H₁₆N₄O₃ · 2H₂O
• Numero CAS: 301836-41-9
• Peso molecolare: 420.42
• Numero MDL: MFCD27976360
• Codice UNSPSC: 51111800
• NACRES: NA.77

Proprietà

• Nome del prodotto: TGF-β RI Kinase Inhibitor VI, SB431542, InSolution, ≥97%
• Livello qualitativo: 100
• Saggio: ≥97% (HPLC)
• Stato: liquid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated (hygroscopic); protect from light
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C
• Stringa SMILE: [nH]1c(nc(c1c4cc5c(cc4)OCO5)c3ncccc3)c2ccc(cc2)C(=O)N
• InChI: 1S/C22H16N4O3/c23-21(27)13-4-6-14(7-5-13)22-25-19(20(26-22)16-3-1-2-10-24-16)15-8-9-17-18(11-15)29-12-28-17/h1-11H,12H2,(H2,23,27)(H,25,26); FHYUGAJXYORMHI-UHFFFAOYSA-N

Descrizione

Descrizione generale

A cell-permeable triarylimidazole compound that is shown to effectively inhibit cellular Smad2 phosphorylation (>90% inhibition by 10 µM inhibitor) upon vector-mediated expression of constitutively active ALK4, ALK5, or ALK7 in NIH 3T3 cells, while exhibiting little effect against Smad1 phosphorylation by other members of type I receptors for TGF-β in NIH 3T3 cultures expressing active ALK1, 2, 3, or 6. When tested directly in cell-free kinase assays, SB431542 is demonstrated to potently inhibit the activity of ALK4 and ALK5 (IC₅₀ = 140 nM and 94 nM, respectively) with no or much reduced potency toward a panel of 24 other kinases (IC₅₀ ≥10 µM in the presence of 10 µM ATP), including ALK2 and ALK6. Reported to improve the efficiency of 4-TF-induced human iPSCs generation from fibroblast cultures by >200-fold when used together with PD0325901 (Cat. No. 444966) and Thiazovivin (Cat. No. 420220).

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Irritant (B)

Stato fisico

A 100 mM (5 mg/119 µl) solution of SB431542 (Cat. No. 616461) in DMSO.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C).

Altre note

Ikushima, H., et al. 2009. Cell Stem Cell5, 504.
Lin, T., et al. 2009. Nat. Methods6, 805.
Maherali, N. and Hochedlinger, K., 2009. Curr. Biol.19, 1718.
Callahan, J.F., et al. 2002. J. Med. Chem.45, 999.
Inman, G.J., et al. 2002. Mol. Pharmacol.62, 65.
Laping, N.J., et al. 2002. Mol. Pharmacol.62, 58.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): 188.6 °F - (refers to pure substance)
• Punto d’infiammabilità (°C): 87 °C - (refers to pure substance)