565785
β-Secretase Activity Assay Kit, Fluorogenic
Sinonimo/i:
BACE Activity Assay Kit, Fluorogenic
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About This Item
Codice UNSPSC:
41116133
Prodotti consigliati
![MCA-[Asn670, Leu671]-Amyloid β/A4 Precursor Protein 770 Fragment 667-676-DNP-Lys-Arg-Arg-NH2 ≥90% (HPLC)](/deepweb/assets/sigmaaldrich/product/images/375/511/f63f11a7-86de-45c4-95a0-7afa616bb265/640/f63f11a7-86de-45c4-95a0-7afa616bb265.jpg)
impiego
sufficient for 100 tests
Livello qualitativo
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
avoid repeated freeze/thaw cycles
protect from light
input
sample type tissue extract(s)
sample type purified enzyme(s) (BACE)
sample type cell lysate
Metodo di rivelazione
fluorometric
Temperatura di conservazione
−20°C
Descrizione generale
A sensitive fluorogenic assay kit for the determination of β-secretase (BACE) activity (Excitation max: 335-355 nm; Emission max: 495-501 nm). β-Secretase is a transmembrane aspartyl protease that cleaves membrane-bound amyloid precursor protein.
Componenti
Extraction Buffer, Reaction Buffer, β-Secretase Substrate, β-Secretase Protein (Positive Control), β-Secretase Inhibitor, and a user protocol.
Attenzione
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Principio
The Calbiochem β-Secretase Activity Assay Kit, Fluorogenic, is intended for measuring the β-secretase (BACE) activity in cell lysates, tissue extracts, or purified enzyme preparations.
Nota sulla preparazione
• Active β-Secretase: Reconstitute the lyophilized Active β-Secretase with 10 µl ddH2O. Following reconstitution, aliquot and freeze (-70°C) to avoid loss of activity.
Stoccaggio e stabilità
Upon arrival store the entire contents of the kit at -20°C. Following reconstitution of the Active β-Secretase, aliquot and freeze (-70°C) to avoid loss of activity. Following initial thaw of the kit contents, store the Extraction Buffer and 2X Reaction Buffer at 4°C.
Altre note
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Dam. 1 - Muta. 2 - Skin Irrit. 2
Codice della classe di stoccaggio
10 - Combustible liquids
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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