553326

Ras Superfamily GTPases GTP/GDP Binding Antagonist, CID 1067700

The Ras Superfamily GTPases GTP/GDP Binding Antagonist, CID 1067700 controls the biological activity of Rab7. This small molecule/inhibitor is primarily used for Membrane applications.

• Sinonimo/i: Ras Superfamily GTPases GTP/GDP Binding Antagonist, CID 1067700, 2-(benzoylcarbamothioylamino)-5,5-dimethyl-4,7-dihydrothieno[2,3-c]pyran-3-carboxylic acid, CID1067700, Cdc42 GTPase Inhibitor I, Rab2 GTPase Inhibitor, Rab GTPase Inhibitor, CDC42 Inhibitor I, Rab7 GTPase Inhibitor, Rac1 GTPase Inhibitor, Rac1 Inhibitor, Rac1 GTPase Inhibitor, Rac1 Inhibitor IV, Ras GTPase Inhibitor, 2-(benzoylcarbamothioylamino)-5,5-dimethyl-4,7-dihydrothieno[2,3-c]pyran-3-carboxylic acid, CID1067700, Cdc42 GTPase Inhibitor I, Rab2 GTPase Inhibitor, Rab GTPase Inhibitor, CDC42 Inhibitor I, Rab7 GTPase Inhibitor
• Formula empirica (notazione di Hill): C₁₈H₁₈N₂O₄S₂
• Numero CAS: 314042-01-8
• Peso molecolare: 390.48
• Numero MDL: MFCD01557530
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Livello qualitativo: 100
• Saggio: ≥98% (HPLC)
• Stato: powder
• Potenza: 12.89-19.70 nM K_i
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; protect from light
• Colore: beige
• Solubilità: DMSO: 50 mg/mL
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: O=C(C1=C(NC(NC(C2=CC=CC=C2)=O)=S)SC3=C1CC(C)(C)OC3)O

Descrizione

Descrizione generale

A thiourea-derived small molecule that acts as a competitive inhibitor of nucleotide (BODIPY-GTP and BODIPY-GDP) binding by Rab7 (K_i = 12.89-19.70 nM). Equilibrates rapidly with Rab7 without affecting the rate of release of bound BODIPY-GTP or BODIPY-GDP. However, it exhibits a slightly lower potency against BODIPY-GDP bound Rab7 than BODIPY-GTP bound Rab7A. Acts by binding to the nucleotide binding pocket of Rab7. Also shown to inhibit nucleotide binding by mutant forms of Rab7 (Rab7Q67L and Rab7T22N) that are constitutively in the GTP and GDP bound states, with good potency. Also shown to block the nucleotide binding of several other Ras-related GTPases, but with reduced potency.

Azioni biochim/fisiol

Cell permeable: yes

Primary Target
Rab7

Reversible: yes

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Standard Handling (A)

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Altre note

Agola, J. O., et al. 2012. ACS Chem Bio.7, 1095.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable