5.00501

iPSC Induction Enhancer II, OAC1

• Sinonimo/i: iPSC Induction Enhancer II, OAC1, Induced Pluripotent Stem Cells Induction Enhancer II, Oct4-Activating Compound 1, N-(1H-Pyrrolo[2,3-c]pyridin-5-yl)benzamide
• Formula empirica (notazione di Hill): C₁₄H₁₁N₃O
• Numero CAS: 300586-90-7
• Peso molecolare: 237.26
• Codice UNSPSC: 12352200

Proprietà

• Saggio: ≥99% (HPLC)
• Livello qualitativo: 100
• Stato: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; protect from light
• Colore: off-white
• Solubilità: DMSO: 100 mg/mL
• Temperatura di conservazione: 2-8°C
• InChI: 1S/C14H11N3O/c18-14(10-4-2-1-3-5-10)17-13-8-11-6-7-15-12(11)9-16-13/h1-9,15H,(H,16,17,18); HWJRIFZDXJKJJN-UHFFFAOYSA-N

Descrizione

Descrizione generale

A cell-permeable pyrrolopyridinyl-benzamide compound that is reported to enhance the efficiency of 4F/4TF- (OSKM; Oct44, Sox2, Klf4, c-Myc) driven iPSC generation from MEFs cultured in either feeder cell-containing ESC media (induction efficiency 20 d post viral transduction = 0.43% vs. 0.186%, respectively, with or without 1 µM OAC1 treatment from d2 to d20) or in feeder-/serum-free iSF1 media (efficiency 10 d post viral transduction = 2.75% vs. 0.68%, respectively, with or without 1 µM OAC1 treatment from d2 to d10). OCA1 is shown to increase cellular Oct4, Nanog, Sox2, and Tet1 mRNA levels via a yet unidentified mechanism, although OAC1 is reported not to affect Oct4 promoter methylation, cellular Wnt signaling, or p53 and p21 mRNA levels. Six other iPSC reprogramming improving reagents, AM580, AzaC, BIX-01294 (Cat. No. 382190), Tranylcypromine (Cat. No. 616431), VPA (Cat. No. 676380), and Vitamin C (Cat. No. 1813), in comparison, do not exhibit Oct4 and Nanog promoters enhancing activity, while BIO (Cat. No. 361550) is demonstrated to be 3.8- and 6-fold less potent than OAC1, respectively, in inducing Oct4 and Nanog transcription (in human ESCs-based reporter assays).

A cell-permeable pyrrolopyridinyl-benzamide compound that is reported to enhance the efficiency of 4F/4TF-induced iPSC generation from MEFs cultured in either feeder cell-containing ESC media or in feeder-/serum-free iSF1 media (1 µM OAC1 treatment starts 48 h after viral transduction). OCA1 is shown to increase cellular Oct4, Nanog, Sox2, and Tet1 mRNA levels via a yet unidentified mechanism, although OAC1 is reported not affect Oct4 promoter methylation, cellular Wnt signaling, or p53 and p21 mRNA levels. Six other iPSC reprogramming improving reagents, AM580, AzaC, BIX-01294 (Cat. No. 382190), Tranylcypromine (Cat. No. 616431), VPA (Cat. No. 676380), and Vitamin C (Cat. No. 1813), in comparison, do not exhibit Oct4 and Nanog promoters enhancing activity, while BIO (Cat. No. 361550) is demonstrated to be 3.8- and 6-fold less potent than OAC1, respectively, in inducing Oct4 and Nanog transcription.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Azioni biochim/fisiol

Cell permeable: yes

Reversible: yes

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Standard Handling (A)

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Use only fresh DMSO for reconstitution.

Altre note

Li, W., et al. 2012. Proc. Natl. Acad. Sci. USA109, 20853.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable