TRANSRTRO
Roche
Transcriptor Reverse Transcriptase
About This Item
Prodotti consigliati
Ricombinante
expressed in E. coli
Livello qualitativo
Saggio
≥90% (SDS-PAGE)
Forma fisica
liquid
impiego
sufficient for 25 reactions
sufficient for 50 reactions
sufficient for 500 reactions
Attività specifica
0.05 U/mg
Caratteristiche
dNTPs included: no
hotstart: no
Produttore/marchio commerciale
Roche
Confezionamento
pkg of 200 reactions (03531287001)
pkg of 25 reactions (03531317001)
pkg of 50 reactions (03531295001)
tecniche
RT-PCR: suitable
RT-qPCR: suitable
Colore
colorless
pH
7.2
Solubilità
water: miscible
Compatibilità
suitable for molecular biology
N° accesso UniProt
applicazioni
genomic analysis
life science and biopharma
Metodo di rivelazione
probe-based
Attività estranea
DNase activity, none detected
Nicking activity, none detected
RNase activity, none detected
Temperatura di conservazione
−20°C (−15°C to −25°C)
Descrizione generale
Applicazioni
- Synthesis of first-strand cDNA for use in subsequent amplification reactions
- RT-PCR of GC-rich RNA templates
- Cy3, Cy5, DIG, biotin, and aminoallyl labeling during cDNA synthesis
- Retrieving and cloning the 5′ and 3′ termini of mRNA by RACE
- Generation of cDNA libraries with large inserts
- Dideoxy DNA sequencing
- RNA sequencing
- 3′-end labeling of DNA fragments
- Generation of single-stranded probes for genomic footprints
- In the reverse transcription of RNA from human Papillomavirus E6, cortical and striatal tissues, muscle biopsies of Becker muscular dystrophy samples and miRNA stem-loop from oocytes and human cerebral microvascular endothelial cells (hCMVECs)
Azioni biochim/fisiol
Caratteristiche e vantaggi
- Achieve high sensitivity in two-step RT-PCR.
- Obtain more full-length transcripts - up to 14 kb.
- Reverse transcribe difficult templates.
- Efficiently label cDNA.
Componenti
- Transcriptor Reverse Transcriptase, in storage buffer
- Transcriptor RT buffer, 5x concentrated
Qualità
- with a conventional thermal cycler to detect a 10-kb fragment from the human dystrophin gene, starting from human skeletal muscle total RNA
- with the LightCycler® Instrument to detect 5 x 102 to 5 x 106 copies of in vitro-transcribed human PBGD RNA. The results are defined in fixed crossing points and fixed fluorescence intensity.
Nota sulla preparazione
Transcription of long, rare, or difficult RNA targets
Transcriptor Reverse Transcriptase is recommended for RT-PCR of:
- long targets, because it can transcribe up to 14 kb RNA templates
- rare targets, because it has high sensitivity
- GC-rich targets, because it can operate at high temperatures (up to +65oC) to eliminate problems associated with extensive secondary structure
Labeling for many applications
Transcriptor Reverse Transcriptase is also recommended for preparing labeled cDNA, since it accepts a wide variety of modified nucleotides (including Cy3-, Cy5-, DIG-, Biotin-, or aminoallyl-labeled dNTPs).
Reaction requirements
Transcriptor accepts ssRNA and ssDNA templates and requires a primer for transcription.
Stoccaggio e stabilità
Altre note
Note legali
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
Certificati d'analisi (COA)
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