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407707

Sigma-Aldrich

PhosphoDetect Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb

liquid, Calbiochem®

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Origine biologica

rabbit

Livello qualitativo

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

liquid

contiene

≤0.1% sodium azide as preservative

Reattività contro le specie

human

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze
avoid repeated freeze/thaw cycles

Isotipo

IgG

Condizioni di spedizione

wet ice

Temperatura di conservazione

−70°C

modifica post-traduzionali bersaglio

phosphorylation (pTyr1162/pTyr1163)

Informazioni sul gene

human ... INSR(3643)

Descrizione generale

Note: 1 T = 1 test. Sufficient for 10 Western mini blots.
Rabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the insulin receptor (IR) protein phosphorylated at Tyr1162/1163 and insulin-like growth factor 1 receptor (IGF-1R) protein phosphorylated at Tyr1162/1163.
Recognizes the ~95 kDa β subunit of the insulin receptor protein phosphorylated at Tyr1162 and Tyr1163 in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr1135 and Tyr1136.
This PhosphoDetect Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb is validated for use in ELISA, WB, ICC for the detection of Insulin Receptor (pTyr1162/1163).

Immunogeno

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1162/1163 phosphorylation sites of human insulin receptor

Applicazioni

ELISA (see comments)

Immunoblotting (1:1000)

Immunocytochemistry (see comments)

Attenzione

Toxicity: Standard Handling (A)

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C).

Altre note

Bevan, P. 2001. J. Cell Sci.114, 1429.
Ottensmeyer, F.P., et al. 2000. Biochemistry39, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest.103, 931.
Schmid, E., at al. 1998. FASEB J.12, 863.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem.262, 15341.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA83, 328.
White, M.F. and Kahn, C.R. 1986. The Enzymes17, 247.
Ebina, Y., et al. 1985. Cell40, 747.
Ganguly, S., et al. 1985. Current Topics in Cellular Regulation27, 83.
Grunberger, G., et al. 1984. Science223, 932.
The immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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