344289
Freund′s Complete Adjuvant, Modified, Mycobacterium butyricum
This Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum is validated for use in Immunization.
Sinonimo/i:
Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum
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About This Item
Prodotti consigliati
Livello qualitativo
Forma fisica
liquid
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
Colore
clear to slightly cloudy amber
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
Descrizione generale
1 Set (10 x 10 ml) each containing yellow, oily, bacterial suspension.
Contains 10 vials per set, each containing 10 ml of Freund′s complete adjuvant homogenized in the following proportions by weight: 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier), and 0.1% Mycobacterium butyricum dry cells.
Applicazioni
Immunization
Attenzione
Toxicity: Standard Handling (A)
Stato fisico
Yellow, oily, bacterial suspension.
Altre note
Composition
The Freund′s Adjuvant set contains 10 identical ampules, each with 10 ml of Freund′s Complete Adjuvant homogenized in the following preparations by weight:
85% Drakeol 5 NF
15% Arlacel A, mannide monooleate emulsifier
0.1% Mycobacterium butyricum dry cells
Sample Preparation
1. Withdraw contents of ampule using a 10 ml syringe with a 1 inch 21 guage needle. Add to a 50 ml vial, preferably with a narrow neck.
2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible. Draw the solution into the syringe.
3. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
4.Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Mixture may seem to thicken as the emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
The Freund′s Adjuvant set contains 10 identical ampules, each with 10 ml of Freund′s Complete Adjuvant homogenized in the following preparations by weight:
85% Drakeol 5 NF
15% Arlacel A, mannide monooleate emulsifier
0.1% Mycobacterium butyricum dry cells
Sample Preparation
1. Withdraw contents of ampule using a 10 ml syringe with a 1 inch 21 guage needle. Add to a 50 ml vial, preferably with a narrow neck.
2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible. Draw the solution into the syringe.
3. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
4.Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Mixture may seem to thicken as the emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Asp. Tox. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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