341006

EDAC, Hydrochloride

EDAC HCl is a water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis.

• Sinonimo/i: EDAC, Hydrochloride, EDCI, 1-Ethyl-3-(3ʹ-dimethylaminopropyl)carbodiimide, HCl
• Formula empirica (notazione di Hill): C₈H₁₇N₃ · xHCl
• Numero CAS: 25952-53-8
• Peso molecolare: 155.24 (free base basis)
• Numero MDL: MFCD00012503
• Codice UNSPSC: 41116133
• NACRES: NA.21

Proprietà

• Livello qualitativo: 200
• Descrizione: RTECS - FF2200000
• Saggio: ≥98% (titration)
• Stato: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated
• Colore: white
• Solubilità: aqueous buffer: 2-5 mg/mL; water: 2-5 mg/mL
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C
• Stringa SMILE: Cl.N(CCCN(CC)C#N)(C)C
• InChI: 1S/C8H17N3.ClH/c1-4-11(8-9)7-5-6-10(2)3;/h4-7H2,1-3H3;1H; FDXPUDRRFDHONO-UHFFFAOYSA-N

Descrizione

Descrizione generale

Molar absorptivity (~212 nm; 50 mM phosphate buffer, pH 8):≥6800 M^-1cm^-1.

Water-soluble carbodiimide derivative. Used to modify NMDA receptors. Also useful for conjugating haptens to proteins or polypeptides.

Water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis. The major advantage of EDAC coupling is the easy removal of excess reagent and the corresponding urea by washing with dilute acid or water. Carbodiimides catalyze the formation of amide bonds, carboxylic acids, and amines by activating the carboxylate to form an O-acylurea. This intermediate can be attacked by an amine directly to form an amide. EDAC is released as a soluble urea derivative.

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Irritant (B)

Nota sulla preparazione

Avoid amine containing buffers.

Ricostituzione

Unstable in solution. Reconstitute just prior to use.

Altre note

Chazot, P.L., et al. 1993. Biochem. Pharmacol. 45, 605.
Richardson, A., et al. 1992. Biochem. Pharmacol. 43, 1415.
Taniuchi, M., et al. 1986. Proc. Natl. Acad. Sci. USA83, 1950.
Chase, J.W., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5480.
Williams, A., et al. 1981. J. Am. Chem. Soc.103, 7090.
Yamada, H., et al. 1981. Biochemistry20, 4836.
Thomas, J.O., et al. 1978. J. Mol. Biol.123, 149.
Ozawa, H. 1970. Biochemistry9, 2158.
Kopple, K.D., et al. 1962. J. Am. Chem. Soc.84, 4457.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS06,GHS08,GHS09
• Avvertenze: Danger
• Indicazioni di pericolo: H302,H311,H315,H317,H373,H410
• Consigli di prudenza: P260 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P314
• Classi di pericolo: Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
• Organi bersaglio: Stomach,large intestine,lymph node
• Codice della classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable