324740

Elastase Substrate V, Fluorogenic

≥95% (HPLC), powder, lyophilized solid, elastase substrate, Calbiochem^®

• Sinonimo/i: Elastase Substrate V, Fluorogenic, MeOSuc-AAPV-AMC
• Formula empirica (notazione di Hill): C₃₁H₄₁N₅O₉
• Numero CAS: 72252-90-5
• Peso molecolare: 627.69
• Numero MDL: MFCD00077135
• Codice UNSPSC: 12352200
• NACRES: NA.25

Proprietà

• product name: Elastase Substrate V, Fluorogenic, Elastase Substrate V, Fluorogenic, CAS 72252-90-5, is a sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM).
• Livello qualitativo: 200
• Saggio: ≥95% (HPLC); ≥95% (TLC)
• Forma fisica: lyophilized solid; powder
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated; protect from light
• Colore: white to off-white
• Solubilità: ethanol: 1 mg/mL; DMF: 5 mg/mL; DMSO: 5 mg/mL
• Fluorescenza: λ_ex 360-380 nm; λ_em 430-460 nm
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C
• InChI: 1S/C31H41N5O9/c1-16(2)27(34-20-9-10-21-17(3)14-26(39)45-23(21)15-20)30(42)35-29(41)22-8-7-13-36(22)31(43)19(5)33-28(40)18(4)32-24(37)11-12-25(38)44-6/h9-10,14-16,18-19,22,27,34H,7-8,11-13H2,1-6H3,(H,32,37)(H,33,40)(H,35,41,42)/t18-,19-,22-,27-/m0/s1; NHMQNKPYBPDCAY-NFHWZJRKSA-N

Descrizione

Descrizione generale

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte (K_m = 140 µM; K_cat/K_m = 120,000 s^-1 M^-1) and porcine pancreatic elastase. Not hydrolyzed by cathepsin G. Excitation max.: ~380 nm; emission max.: ~460 nm.

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM; k_cat/K_m = 120,000 M^-1 s^-1). Not hydrolysed by cathepsin G.

Azioni biochim/fisiol

Cell permeable: no

Km = 140 µM; kcat/Km = 120,000 M⁻¹ s⁻¹as substrates for human leukocyte and porcine pancreatic elastase

Primary Target
Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase

Product does not compete with ATP.

Reversible: no

Attenzione

Toxicity: Standard Handling (A)

Sequenza

MeOSuc-Ala-Ala-Pro-Val-AMC

Ricostituzione

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.

Altre note

Barrett, A.J. 1981. Methods Enzymol. 80, 581.
Castillo, M.J., et al. 1979. Anal. Biochem.99, 53.
Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable