164599

Aminopeptidase N, Rat

• Sinonimo/i: APN/CD13, aminopeptidase N
• Classificazione EC (Enzyme Commission): 3.4.11.2 (BRENDA, IUBMB)
• Codice UNSPSC: 12352202
• NACRES: NA.54

Proprietà

• Saggio: ≥90% (SDS-PAGE)
• Livello qualitativo: 100
• Forma fisica: liquid
• Attività specifica: ≥100 units/mg protein
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

Native aminopeptidase N purified from rat renal brush-border membranes and cleaved with papain to produce soluble enzyme. A single-chain, integral membrane protease that functions to remove N-terminal amino acids from peptides with broad specificity. Aminopeptidase N is believed to play a role in angiogenesis and elevated levels of aminopeptidase N have been reported in patients with various types of cancer and have been correlated with tumor load. Also involved in the final stages of digestion of small peptides in the intestinal brush border.

Native aminopeptidase N purified from rat renal brush-border membranes and cleaved with papain to produce soluble enzyme. A single-chain, integral membrane protease that, functions with broad specificity to remove N-terminal amino acids from peptides. It has been reported that levels of aminopeptidase N are elevated in cancer subjects and correlate with tumor load, and thus, the enzyme is believed to play a role in angiogenesis.

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol L-Leu-pNA per min at 37°C, pH 7.2

Stato fisico

In 200 mM NaCl, 50 mM Tris-HCl, pH 8.0.

Altre note

Bhagwat, S.V., et al. 2003. Blood101, 1818.
van Hensbergen, Y., et al. 2002. Clin. Cancer Res.8, 3747.
Ishii, K., et al. 2001. Biol. Pharm. Bull.24, 226.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable