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Key Documents

1.52007

Supelco

Chromolith® RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 50 mm x 2 mm HPLC Column

Sinonimo/i:

Chromolith® FastGradient RP-18 endcapped 50-2 HPLC column

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About This Item

Codice UNSPSC:
41115709
NACRES:
SB.52

product name

Chromolith® RP-18 endcapped, L × I.D. 50 mm x 2 mm HPLC column

Materiali

PEEK column

Livello qualitativo

agenzia

suitable for USP L1

Nome Commerciale

Chromolith®

Caratteristiche

endcapped

Confezionamento

1 ea of

Grado di funzionalizzazione

18% Carbon loading

Parametri

200 bar pressure
45 °C max. temp.

tecniche

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

Lungh. × diam. int. colonna

5 cm × 2 mm

Matrice

monolithic silica

Gruppo funzionale matrice

C18 bonding phase

Dimensione pori

1.5 μm macroporosity
13 nm mesoporosity

operating pH range

2-7.5

Tecnica di separazione

reversed phase

Temperatura di conservazione

no temp limit

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Descrizione generale

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® FastGradient RP-18 endcapped 50-2 columns have macropores with 1.5 μm diameter, giving a column efficiency exceeding 100,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® FastGradient RP-18 endcapped 50-2 columns fall under the USP L1 Classification. The column efficiency is comparable to a 3-4 μm particle size fully porous particulate column. Chromolith® FastGradient RP-18 endcapped 50-2 columns are perfectly suitable for LC-MS applications.
The column dimension of 50 mm length and 2 mm I.D. is the best choice for fast separations on any U/HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enable:
  • Rapid separations at very low column back-pressure
  • High matrix tolerance, allowing time and cost savings with simplified workflows
  • Cost-savings due to extended column lifetimes
  • Connection of columns, flow gradients and the use of mobile phases with higher viscosity


The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-2 guard cartridges (3 pieces) [1.52009] with the Chromolith® Guard Cartridge Holder (kit) 5 mm x 2 mm [1.52004].

The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467].

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Risultati analitici

Appearance: passes test
Pressure: ≤ 30 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 100000
Capacity factor (Anthracene): 3.6 - 4.3
Tailing (USP): 0.86 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 2.30 - 2.60

Note legali

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articoli

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.

Contenuto correlato

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.