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Merck
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Documenti fondamentali

14-276-M

Sigma-Aldrich

Akt1/PKBα Protein, active, 15 µg

Active, recombinant full-length human Akt1, containing N-terminal His6 tag, for use in IP-Kinase Assays & Kinase Assays.

Sinonimo/i:

Protein kinase B alpha, Proto-oncogene c-Akt, RAC, RAC-PK-alpha

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About This Item

Codice UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.26

Origine biologica

human

Livello qualitativo

100

Ricombinante

expressed in baculovirus infected Sf21 cells

Stato

liquid

Durata

1 yr

PM

Mw 59.9 kDa

Produttore/marchio commerciale

Upstate®

tecniche

activity assay: suitable (IP-kinase)
activity assay: suitable (kinase)

Solubilità

soluble

N° accesso NCBI

NM_001014431.1
NM_001014432.1
NM_005163.2

N° accesso UniProt

P31749

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... AKT1(207)

Descrizione generale

Research area: CELL SIGNALING

Product Source: Expressed by baculovirus in Sf21 insect cells

Recombinant full-length human Akt1, containing N-terminal His6-tag.

Azioni biochim/fisiol

Protein Target: Akt1/PKBα

Target Sub-Family: AGC

Akt, also known as protein Kinase B (PKB), is a crucial protein involved in various cell signaling pathways. It modifies and controls the activities of numerous proteins involved in a wide range of cellular and physiological processes. Some of these processes include cell survival, apoptosis, cell proliferation, transcription, cell migration, and metabolism. Akt also helps in influencing angiogenesis, and normal development of embryos. Akt regulates cell survival through the phosphorylation of mitogen-activated protein kinase kinase kinase 5 (MAP3K5), which is involved in the cellular apoptotic response. The Akt occupies a pivotal position in the phosphoinositide 3-kinase-protein kinase B-mammalian target of rapamycin (PI3K-PKB-mTOR) signaling pathway. Genetic mutations occurring upstream of Akt in this cascade can result in uncontrolled cell proliferation and the development of cancer. In addition abberent Akt activation is associated with the development of type-2 diabetes.

Confezionamento

Also available in 250μg size, please inquire for pricing and availability.

Qualità

Routinely evaluated by phosphorylation of Crosstide. Please refer to the Certificate of Analysis for more information.

Stato fisico

Ni2+/NTA and glutathione agarose
Supplied in 50mM Tris/HCl pH7.5, 150mM NaCl, 150mM imidazole, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.07% 2-mercaptoethanol, 0.02% Brij 35, 270mM sucrose. Frozen solution.

Stoccaggio e stabilità

1 year at -70°C

Altre note

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pittogrammi

Health hazardExclamation mark
GHS08,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H315,H317,H319,H360D

Classi di pericolo

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - Skin Sens. 1

Codice della classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe di pericolosità dell'acqua (WGK)

WGK 2

Elenchi normativi

Forniamo informazioni su eventuali restrizioni prevalentemente per i prodotti chimici. Per altre tipologie di prodotto siamo in grado di fornire soltanto informazioni limitate. Nessuna segnalazione significa che nessuno dei componenti è citato in un elenco. È dovere dell’utilizzatore assicurarsi che il prodotto venga impiegato in maniera sicura e a norme di legge.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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A common phosphate binding site explains the unique substrate specificity of GSK3 and its inactivation by phosphorylation
Frame, S., et al
Molecular Cell, 7, 1321-1327 (2001)
Stoichiometry of site-specific protein phosphorylation estimated with phosphopeptide-specific antibodies.
Miinea, Cristinel P and Lienhard, Gustav E
Biotechniques, 34, 828-831 (2003)
B Bauer et al.
The Journal of biological chemistry, 276(34), 31627-31634 (2001-06-19)
Protein kinase C theta (PKC theta) is known to induce NF-kappa B, an essential transcriptional element in T cell receptor/CD28-mediated interleukin-2 production but also T cell survival. Here we provide evidence that PKC theta is physically and functionally coupled to
Madhavi J Rane et al.
The Journal of biological chemistry, 278(30), 27828-27835 (2003-05-13)
Activation of the serine-threonine kinase Akt by cytokines, chemokines, and bacterial products delays constitutive neutrophil apoptosis, resulting in a prolonged inflammatory response. We showed previously that Akt exists in a signaling complex with p38 MAPK, MAPK-activated protein kinase-2 (MAPKAPK-2), and
P Cohen et al.
FEBS letters, 410(1), 3-10 (1997-06-23)
The initial steps in insulin signal transduction occur at the plasma membrane and lead to the activation of phosphatidylinositide (PtdIns) 3-kinase and the formation of PtdIns(3,4,5,)P3 in the inner leaflet of the plasma membrane which is then converted to PtdIns(3,4)P2
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