06-1398
Anti-CDK5RAP2 Antibody
from rabbit, purified by affinity chromatography
Sinonimo/i:
CDK5 regulatory subunit-associated protein 2, CDK5 activator-binding protein C48, Centrosome-associated protein 215
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Purificato mediante
affinity chromatography
Reattività contro le specie
human
tecniche
immunocytochemistry: suitable
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... CDK5RAP2(55755)
Descrizione generale
Also known as Cep215, CDK5RAP2 is involved in a variety of cellular processes. It serves as a potential regulator of CDK5 activity through its interaction with CDK5R1. It acts as a negative regulator of centriole disengagement, which maintains centriole engagement and cohesion. It is involved in the regulation of mitotic spindle orientation. Additionally, this protein mediates spindle checkpoint activation by serving as a transcriptional regulator of the MAD2 and BUBR1 promoter. In conjuction with MAPRE1, CDK5RAP2 promotes microtubule polymerization, bundle formation, and growth at the plus ends. It also plays a role in DNA damage-induced G2 cell cycle checkpoint. CDK5RAP2 is known to be mutated in primary microcephaly, a neuodevelopmental disorder that is characterized by a decrease in brain size.
Specificità
Other Homologies: Rat (83% sequence homology) and Mouse (82% sequence homology).
Immunogeno
MBP-tagged recombinant protein corresponding to human CDK5RAP2.
Applicazioni
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected CDK5RAP2 in RPE1 cells. (Barr, A.R., et al. (2010). J Cell Biol. 189(1):23-39.)
Use Anti-CDK5RAP2 Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect CDK5RAP2 also known as CDK5 regulatory subunit-associated protein 2, CDK5 activator-binding protein C48, Centrosome-associated protein 215.
Qualità
Evaluated by Western Blot in 10 µg of HeLa cell lysate.
Western Blot Analysis: A 1:2,500 dilution of this antibody dectected CDK5RAP2 in 10µg of HeLa cell lysate.
Western Blot Analysis: A 1:2,500 dilution of this antibody dectected CDK5RAP2 in 10µg of HeLa cell lysate.
Descrizione del bersaglio
~220 kDa observed. Uniprot.org states four isoforms produced by alternative splicing, which may be observed at a molecular weight of ~216 kDa, ~211 kDa, ~210 kDa, and ~206 kDa in some cell lysates.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Joo-Hee Sir et al.
The Journal of cell biology, 203(5), 747-756 (2013-12-04)
Most animal cells contain a centrosome, which comprises a pair of centrioles surrounded by an ordered pericentriolar matrix (PCM). Although the role of this organelle in organizing the mitotic spindle poles is well established, its precise contribution to cell division
Andrew Muroyama et al.
The Journal of cell biology, 213(6), 679-692 (2016-06-15)
Differentiation induces the formation of noncentrosomal microtubule arrays in diverse tissues. The formation of these arrays requires loss of microtubule-organizing activity (MTOC) at the centrosome, but the mechanisms regulating this transition remain largely unexplored. Here, we use the robust loss
Alexis R Barr et al.
The Journal of cell biology, 189(1), 23-39 (2010-04-07)
The centrosomal protein, CDK5RAP2, is mutated in primary microcephaly, a neurodevelopmental disorder characterized by reduced brain size. The Drosophila melanogaster homologue of CDK5RAP2, centrosomin (Cnn), maintains the pericentriolar matrix (PCM) around centrioles during mitosis. In this study, we demonstrate a
Tatsuo Miyamoto et al.
Human molecular genetics, 26(22), 4429-4440 (2017-10-04)
Primary microcephaly (MCPH) is an autosomal recessive disorder characterized by congenital reduction of head circumference. Here, we identified compound heterozygous mutations c.731 C > T (p.Ser 244 Leu) and c.2413 G > T (p.Glu 805 X) in the WDR62/MCPH2 gene, which encodes the mitotic centrosomal protein
Shinya Ohta et al.
PloS one, 10(11), e0142798-e0142798 (2015-11-13)
The centrosome-associated C1orf96/Centriole, Cilia and Spindle-Associated Protein (CSAP) targets polyglutamylated tubulin in mitotic microtubules (MTs). Loss of CSAP causes critical defects in brain development; however, it is unclear how CSAP association with MTs affects mitosis progression. In this study, we
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