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Key Documents

05-801

Sigma-Aldrich

Anti-trimethyl-Histone H3 (Lys36) Antibody, clone MC86, rabbit monoclonal

clone MC86, Upstate®, from rabbit

Sinonimo/i:

H3K36me3, Histone H3 (tri methyl K36)

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

MC86, monoclonal

Reattività contro le specie

chicken

Produttore/marchio commerciale

Upstate®

tecniche

ChIP: suitable
dot blot: suitable
inhibition assay: suitable (peptide)
western blot: suitable

Isotipo

IgG

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

trimethylation (Lys36)

Specificità

Broad species cross-reactivity is expected.
Recognizes Histone H3 trimethylated on Lysine 36.

Immunogeno

peptide containing the sequence GVme3KKP, in which me3K corresponds to trimethyl lysine 36 of human histone H3

Applicazioni

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-trimethyl-Histone H3 (Lys36) Antibody, clone MC86 (rabbit monoclonal antibody) validated in DB, WB, PIA to detect trimethyl-Histone H3 (Lys36) also known as H3K36me3, Histone H3 (tri methyl K36).

Qualità

routinely evaluated by immunoblot on chicken Core Histones (Catalog #13-107); did not detect unmodified recombinant Histone H3 (Catalog #14-411)

Descrizione del bersaglio

~17 kDa

Stato fisico

Format: Purified
Protein A Purified immunoglobulin in a solution containing 0.07M Tris-glycine, 0.105M NaCl, pH 7.4, 0.035% sodium azide, 30% glycerol.
Protein A purified

Stoccaggio e stabilità

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Risultati analitici

Control
Acid extracted proteins from HeLa cells

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Jian Zhang et al.
The Plant journal : for cell and molecular biology, 64(4), 604-617 (2010-09-09)
MADS-box transcription factors are known for their roles in plant growth and development. The regulatory mechanisms of spatial and temporal specific expression of MADS-box genes and the function of MADS-box genes in other biological processes are still to be explored.
The Iws1:Spt6:CTD complex controls cotranscriptional mRNA biosynthesis and HYPB/Setd2-mediated histone H3K36 methylation.
Yoh, SM; Lucas, JS; Jones, KA
Genes & Development null
Mass spectrometry analysis of the variants of histone H3 and H4 of soybean and their post-translational modifications.
Wu, T; Yuan, T; Tsai, SN; Wang, C; Sun, SM; Lam, HM; Ngai, SM
BMC plant biology null
Jun Wang et al.
Nature plants, 6(7), 823-837 (2020-06-24)
Histone demethylation is crucial for proper chromatin structure and to ensure normal development, and requires the large family of Jumonji C (JmjC)-containing demethylases; however, the molecular mechanisms that regulate the substrate specificity of these JmjC-containing demethylases remain largely unknown. Here
Harry G Leitch et al.
Nature structural & molecular biology, 20(3), 311-316 (2013-02-19)
Naive pluripotent embryonic stem cells (ESCs) and embryonic germ cells (EGCs) are derived from the preimplantation epiblast and primordial germ cells (PGCs), respectively. We investigated whether differences exist between ESCs and EGCs, in view of their distinct developmental origins. PGCs

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