05-797R
Anti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonal
clone AW39R, Upstate®, from rabbit
Sinonimo/i:
Erk1, Erk2, p44-MAPK, p42-MAPK, MAPK2, PRKM2, p41mapk, PRKM1, p44, ERK2, P42MAPK, ERK, p42, MAPK1, ERT1, ERT2, Mitogen-activated protein kinase, MAP Kinase 2, MAP Kianse 1, MAPK
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
AW39R, monoclonal
Reattività contro le specie
human, mouse, rat
Produttore/marchio commerciale
Upstate®
tecniche
multiplexing: suitable
western blot: suitable
Isotipo
IgG
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
phosphorylation ((pThr202/pTyr204), (pThr185/pTyr187))
Informazioni sul gene
human ... MAPK3(5595)
mouse ... Mapk3(26417)
rat ... Mapk3(50689)
Descrizione generale
Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).
Specificità
Recognizes Erk 1 & 2 only when dually phosphorylated on its TxY activation motif.
Immunogeno
Epitope: pTEpY in the activation loop
Phosphorylated peptide corresponding to amino acids surrounding the pTEpY motif in the activation loop of human phospho-Erk1/2, in which the Thr and Tyr residues are phosphorylated
Applicazioni
Anti-phospho-Erk1/2 (Thr202/Tyr204 Antibody, Thr185/Tyr187), recombinant clone AW39R detects level of phospho-Erk1/2 (Thr202/Tyr204 & has been published & validated for use in Mplex & WB.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
Kinases & Phosphatases
MAP Kinases
Kinases & Phosphatases
Qualità
Routinely evaluated by immunoblot on lysate from PDGF-treated NIH/3T3 cells
Descrizione del bersaglio
Erk1 (p44) (~44 kDa) and Erk2 (p42) (~42 kDa).
Linkage
Replaces: 05-797
Stato fisico
Format: Purified
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Protein A purified
Stoccaggio e stabilità
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Risultati analitici
Control
PDGF-treated NIH/3T3 cell lysates
PDGF-treated NIH/3T3 cell lysates
Note legali
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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