05-623-AF488

Anti-RNA polymerase II Antibody, clone CTD4H8, Alexa Fluor^™ 488 Conjugate

clone CTD4H8, from mouse, ALEXA FLUOR^™ 488

• Sinonimo/i: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Coniugato: ALEXA FLUOR^™ 488
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: CTD4H8, monoclonal
• Reattività contro le specie: human, mouse
• Reattività contro le specie (prevista in base all’omologia): yeast (based on 100% sequence homology), rat (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable
• Isotipo: IgG1
• N° accesso NCBI: NP_000928.1
• N° accesso UniProt: P24928
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... POLR2A(5430) ; mouse ... Polr2A(20020) ; rat ... Polr2A(363633)

Descrizione

Descrizione generale

DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Applicazioni

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in HeLa cells.
The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.

This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor 488 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.

Qualità

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in A431 cells.

Descrizione del bersaglio

~210-220 kDa observed

Altre note

Concentration: Please refer to lot specific datasheet.

Note legali

ALEXA FLUOR is a trademark of Life Technologies

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable