05-623-Z

Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free

clone CTD4H8, from mouse

• Sinonimo/i: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: CTD4H8, monoclonal
• Reattività contro le specie: mouse, yeast, rat, human
• tecniche: ChIP: suitable (ChIP-seq); immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_000928
• N° accesso UniProt: P24928
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... POLR2A(5430) ; mouse ... Polr2A(20020) ; rat ... Polr2A(363633)

Descrizione

Descrizione generale

RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Immunogeno

Linear peptide corresponding to human RNA polymerase II.

Applicazioni

Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free is a highly specific mouse monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting, ICC & ChIP-seq.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western Blotting Analysis: 1 µg/mL from a representative lot detected RNA polymerase II in 10 µg of HeLa cell lysate.

Immunocytochemistry Analysis: A 1:50 and 1:250 dilution from a representative lot detected RNA polymerase II in HeLa and A431 cells, respectively.

Qualità

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected RNA polymerase II in 10 µg of NIH/3T3 cell lysate.

Descrizione del bersaglio

~220 kDa observed

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. (This product is Ascites Free.)

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable