05-1406

Anti-Phospholipase A2 Antibody, clone CH-7

clone CH-7, from mouse

• Sinonimo/i: Phospholipase A2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: CH-7, monoclonal
• Reattività contro le specie: rat, human, mouse
• tecniche: ELISA: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG2b
• N° accesso NCBI: NM_000928.2
• N° accesso UniProt: P04054
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... PLA2G10(8399)

Descrizione

Descrizione generale

Phospholipases hydrolyze phosphoglycerides to form smaller compounds which often serve as critical second messengers. Phospholipase A2 (PLA2) hydrolyzes phosphatidylcholine to produce lysophosphatidylcholine, a second messenger involved in cell proliferation, adhesion, T-cell activation, and smooth muscle cell contractility. PLA2 isoforms include membrane-associated, Ca2+-independent forms, cytosolic, Ca2+-dependent forms, and secretory forms. PLC catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate, to generate inositol (1,4,5) trisphosphate (IP3) and diacylglycerol (DAG), leading to Ca2+ release and PKC activation, respectively. At least four types of PLC have been identified. PLCβ, is activated by G-Protein-Coupled Receptors (GPCRs) and heterotrimeric G-Proteins. Mechanisms of PLCδ activation are unclear, but may also involve GPCRs. PLCγ, is activated by receptor tyrosine kinases, and appears essential for signaling by the PDGF Receptor. PLCε is a Guanine Nucleotide Exchange factor (GEF) for the small G-Protein Ras. PLD activity is up-regulated when cells are stimulated by hormones, growth factors or other extracellular signals. Two distinct PLDs, PLD1 and PLD2, have been identified. PLD hydrolyzes phosphatidylcholine to release phosphatidic acid, which is associated with a number of cellular responses including regulated secretion, mitogenesis and cytoskeletal changes.

Specificità

This antibody recognizes PLA2.

Immunogeno

Recombinant cPLA2

Applicazioni

Anti-Phospholipase A2 Antibody, clone CH-7 detects level of Phospholipase A2 & has been published & validated for use in WB, ELISA, IH(P).

ELISA: A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by the end user.

Immunohistochemistry(paraffin): Representative images from a previous lot.
Optimal Staining of PLA2 Monoclonal Antibody: Colon Cancer

Western Blot: A previous lot of this antibody was used at 1:500-1:1000; antibody recognizes all isoforms, the cPLA. Alpha (85 kDa) typically migrates as 100-110 kDa on reducing westerns; beta migrates as 114 kDa; antibody will also recognize the sPLA at approximately 14-20 kDa.

Research Category
Signaling

Research Sub Category
Lipid Signaling

Qualità

Routinely evaluated by Western Blot on RAW 264 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PLA2 on 10 μg of RAW 264 lysates.

Descrizione del bersaglio

Alpha (85 kDa) typically migrates as 100-110 kDa on reducing westerns; beta migrates as 114 kDa; antibody will also recognize the sPLA at approximately 14-20 kDa

Linkage

Replaces: MAB1668

Stato fisico

Format: Purified

Protein A purified

Purified mouse monoclonal IgG2b in buffer containing 20 mM sodium phosphate, 250 mM sodium chloride, 0.1% sodium azide, pH. 7.6.

Stoccaggio e stabilità

Stable at 2-8°C for 1 year from date of receipt.

Risultati analitici

Control
RAW264 Cell Lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable