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Key Documents

SAB4200757

Sigma-Aldrich

Anti-PLA2R antibody, Mouse monoclonal

clone 12-6-5, purified from hybridoma cell culture

Sinonimo/i:

Anti-180 kDa secretory phospholipase A2 receptor, Anti-C-type lectin domain family 13 member C, Anti-M-type receptor, Anti-PLA2-R, Anti-PLA2R, Anti-Secretory phospholipase A2 receptor

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

primary antibodies

Clone

12-6-5, monoclonal

Forma fisica

buffered aqueous solution

PM

~180 kDa

Reattività contro le specie

human

Concentrazione

~1 mg/mL

tecniche

flow cytometry: suitable
immunoblotting: 1-2 μg/mL using partial recombinant human PLA2R1 protein (predicted ~150kDa)
immunofluorescence: suitable
immunohistochemistry: suitable

Isotipo

IgG2b

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... PLA2R1(22925)

Descrizione generale

Anti-PLA2R antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the 12-6-5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with recombinant fragment of human PLA2R corresponding to extracellular sequence NC3. Secretory phospholipase A2 receptor (PLA2R) is 180 kDa and is a type I transmembrane glycoprotein member of the mannose receptor (MR) family. Large extracellular glycosylated region comprising an N-terminal cysteine-rich domain (CysR), a fibronectin-like type II domain (FnII), and eight to ten C-type lectin-like domains (CTLD).

Immunogeno

Human PLA2R extracellular sequence N-C3 (containing domains N-terminal Cysteine rich-FibII-CTLD1-CTLD2-CTLD3)

Applicazioni

Anti-PLA2R antibody has been used in
  • immunoblotting
  • immunofluorescence
  • flow Cytometry
  • immunohistochemistry

Azioni biochim/fisiol

Secretory phospholipase A2 receptor (PLA2R) acts as a receptor for secretory phosholipase A2 (sPLA2) allowing its removal from circulation, thus regulating its biological effect. PLA2R is also involved in clearance followed by suppression of their potent enzymatic activities of secretory phospholipase A2 (sPLA2) from the blood, including group sPLA2-X and a snake venom sPLA2. The soluble circulating form of PLA2R is constitutively present as an endogenous inhibitor of sPLA2s. PLA2R is expressed in kidney podocytes of normal human glomeruli, in idiopathic membranous nephropathy (IMN) patients PLA2R is colocalized to immune deposits in glomeruli along with IgG4.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Phospholipase A2 receptor: a regulator of biological functions of secretory phospholipase A2
Hanasaki, Kohji and Arita, Hitoshi
Prostaglandins & other lipid mediators, 68, 71-82 (2002)
Membranous nephropathy: integrating basic science into improved clinical management
Cattran D C and Brenchley P E
Kidney International, 91(3), 566-574 (2017)
Bogdan Obrisca et al.
BioMed research international, 2015, 249740-249740 (2015-11-18)
Since the identification of PLA2R (M-type phospholipase A2 receptor) as the first human antigenic target in primary membranous nephropathy (MN), perpetual progress has been made in understanding the pathogenesis of this disease. Accumulating clinical data support a pathogenic role for
Antiphospholipase A2 receptor autoantibodies: a step forward in the management of primary membranous nephropathy
Obrisca B, et al.
BioMed Research International, 2015(3) (2015)
Laurence H Beck et al.
The New England journal of medicine, 361(1), 11-21 (2009-07-03)
Idiopathic membranous nephropathy, a common form of the nephrotic syndrome, is an antibody-mediated autoimmune glomerular disease. Serologic diagnosis has been elusive because the target antigen is unknown. We performed Western blotting of protein extracts from normal human glomeruli with serum

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