04-721

Anti-Dicer1 Antibody, clone 5D12.2

clone 5D12.2, Upstate^®, from mouse

• Sinonimo/i: DCR1, Dicer, HERNA, KIAA0928
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 5D12.2, monoclonal
• Reattività contro le specie: human
• Confezionamento: antibody small pack of 25 μL
• Produttore/marchio commerciale: Upstate^®
• tecniche: western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_085124.2
• N° accesso UniProt: Q9UPY3
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... DICER1(23405)

Descrizione

Descrizione generale

RNA interference (RNAi) is mechanism for post-transcriptional gene silencing that leads to the loss of the activity of the gene. RNAi is activated when long dsRNAs are cleaved by the enzyme Dicer1 (RNase III family of dsRNA-specific endonucleases) into 21-23 nucleotide siRNA (small interfering RNA). These siRNAs bind proteins to form the RISC (RNA-induced silencing complex) which is activated when the double stranded siRNAs are unzipped to single stranded siRNAs. Activated RISC recognizes and binds complementary sequences in mRNA. The mRNA is cleaved and the overall effect is an inhibition of the protein expression and a loss of the gene activity. Dicer1 along with the Argonaute family of proteins (Ago1 and Ago2) share the same domain PIWI and PAZ domain that provide endonuclease activity to RNA-induced silencing complexes (RISC).

Specificità

Human Dicer1

Immunogeno

KLH conjugated synthetic peptide corresponding to amino acids 378-385 of human Dicer1

Applicazioni

Anti-Dicer1 Antibody, clone 5D12.2 is a Mouse Monoclonal Antibody for detection of Dicer1 also known as DCR1, Dicer, HERNA, KIAA0928 & has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

RNA Binding Protein (RBP)

Western blot

Qualità

Routinely evaluated by western blot on lysates from HeLa nuclear extracts.

Descrizione del bersaglio

220 kDa

Stato fisico

Format: Purified

Protein G Chromatography

Stoccaggio e stabilità

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Altre note

feature_concentration_valuePlease refer to lot specific datasheet.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable