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Key Documents

NCXAUCR40

Sigma-Aldrich

Gold Nanospheres

Bare, 40 nm, for Passive Adsorption, Bioready, Bare (Citrate), OD 20, in Aqueous Sodium Citrate

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About This Item

Codice UNSPSC:
12162002
NACRES:
NA.23

Forma fisica

nanospheres

OD

20

Dimensione particelle

40 nm

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Applicazioni

Owing to their plasmonic properties nanoparticles of gold and silver find broad range of applications extending from diagnostics to molecular detection, photothermal and microwave-based therapies, and nanotoxicology. Colloidal silver nanoparticles also find applications in antimicrobial coatings, electronics and photovoltaics, and surface enhanced Raman spectroscopy (SERS) whereas the colloidal gold nanoparticles find applications in lateral flow assays (immunochromatographic assays) and as TEM labels and size standards.

The BioReady gold nanospheres are well suited for passive conjugation of antibodies, proteins, peptides, and other biomolecules.

The BioReady citrate gold nanoparticles have a “naked” particle surface which contains a weakly bound citrate molecule as stabilizer. The citrate is readily displaced in presence of proteins and allows passive adsorption (also referred to as physisorption). The mechanism of adsorption is based on Van der Waals interactions between the proteins (e.g. antibodies) and the surface of the particles. The resulting forces between the antibody and the nanoparticle are influenced by the coupling environment. Our BioReady citrate gold is provided at an optical density (OD) of 20 at pH ~8.0–8.5. A pH titration should be performed to optimize the pH of conjugation.

The BioReady carbonate gold nanoparticles have a “naked” particle surface that contains a weakly bound carbonate molecule as particle stabilizer and, similar to citrate-stabilized gold, the carbonate is readily displaced in the presence of proteins and allows easy passive adsorption. Carbonate is a smaller and less complex molecule and has a lower affinity to the gold nanoparticle surface compared to citrate. This enhances the displaceability of the carbonate molecules allows better conjugation. Our BioReady carbonate gold is provided at an optical density (OD) of 20 at pH ~7.4–8.6. A pH titration should be performed to optimize the pH of conjugation.

Stoccaggio e stabilità

DO NOT FREEZE. Store at 2-8C and away from light when not in use to maximize shelf life. Nanomaterials can be sensitive to high and low pH conditions and high salt concentrations. Use caution when changing environmental conditions as particles may aggregate.

Note legali

Product of nanoComposix, Inc.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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