B0126

Bromophenol Blue

titration: suitable

• Synonym(s): 3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein, Bromophenol Blue (BPB), Bromphenol Blue Sultone Form
• Empirical Formula (Hill Notation): C₁₉H₁₀Br₄O₅S
• CAS Number: 115-39-9
• Molecular Weight: 669.96
• Beilstein: 61698
• EC Number: 204-086-2
• MDL number: MFCD00005875
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891504
• NACRES: NA.47

Properties

• Agency: suitable for SM 2310; suitable for SM 2320
• Quality Level: 200
• form: powder
• technique(s): titration: suitable
• color: faint beige to very dark beige; faint orange to very dark orange
• visual transition interval: 3.0-4.6, yellow-green to blue
• mp: 273 °C
• solubility: methanol: 10 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4
• InChI: 1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
• InChI key: UDSAIICHUKSCKT-UHFFFAOYSA-N

Description

General description

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves