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Merck

HPLC Analysis of Sugars on Ascentis® Si

HPLC Analysis of Sugars on Ascentis® Si application for HPLC

材料

分析柱

产品编号
说明
价格

581512-U

Ascentis® Si HPLC 色谱柱

5 μm particle size, L × I.D. 15 cm × 4.6 mm

標準

产品编号
说明
价格

84097

蔗糖

BioUltra, for molecular biology, ≥99.5% (HPLC)

47287-U

乳糖一水合物

analytical standard

G0350500

D -(+)-葡萄糖

European Pharmacopoeia (EP) Reference Standard

X0200000

D-(+)-木糖

European Pharmacopoeia (EP) Reference Standard

CONDITIONS

column

Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] water; [B] acetonitrile; (15:85, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

说明

分析報告

This application demonstrates the suitability of the Ascentis Si for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Si are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

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