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材料
stainless steel
agency
suitable for USP L20
suitable for USP L59
產品線
TSKgel®
製造商/商標名
Tosoh 0023448
參數
≤0.5 M salt concentration
0-30% aqueous soluble organic solvents
0.35 mL/min flow rate
10-30 °C temp. range
340 bar pressure
技術
HPLC: suitable
長度 × 內徑
30 cm × 4.6 mm
基質
silica particle platform
基質活性組
diol phase
粒徑
2 μm
孔徑
250 Å
pH值範圍
2.5-7.5
分離技術
size exclusion (SEC)
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These columns are designed to be operated with a simple and well established method (sodium phosphate mobile phase, pH 6.8) and are packed for use in both HPLC and UHPLC systems.
These columns are designed to be operated with a simple and well established method (sodium phosphate mobile phase, pH 6.8) and are packed for use in both HPLC and UHPLC systems.
Informative article on size exclusion chromatography columns for biomolecule separations. Using columns packed with sub-2 μm particles provides significant gains in efficiency over SEC columns packed with larger particles.
Informative article on size exclusion chromatography columns for biomolecule separations. Using columns packed with sub-2 μm particles provides significant gains in efficiency over SEC columns packed with larger particles.
实验方案
Bispecific antibodies are a new class of biotherapeutics where one therapeutic agent can target two different antigens. This application note demonstrates a new size exclusion chromatographic method for characterizing these new biotherapeutics by mass spectrometry.
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