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product name
SUPELCOSIL™ LC-Si HPLC 柱, 5 μm particle size, L × I.D. 30 cm × 4 mm
agency
suitable for USP L3
品質等級
製造商／商標名
SUPELCOSIL™
參數
≤70 °C temp. limit
400 bar pressure (5801 psi)
技術
HPLC: suitable
長度 × 內徑
30 cm × 4 mm
表面積
170 m2/g
基質
silica gel, spherical particle platform
基質活性組
silica phase
粒徑
5 μm
孔徑
120 Å pore size
pH值範圍
2-7.5
應用
food and beverages
相容性
mode of use normal phase HPLC
分離技術
hydrophilic interaction (HILIC)
normal phase
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一般說明
与正相硅胶柱配合使用的流动相，通常包含己烷和庚烷等烃类溶剂，并混合有相对较少的强极性溶剂。非极性化合物将首先从柱上洗脱，而极性溶质与硅胶表面上的硅烷醇基团具有较强的相互作用。硅胶的极性选择性对于毒枝菌素的分离非常有用。由于硅胶的低运行成本以及相对于反相柱反压较低，通常用于制备色谱中。硅胶是有机化学家最常用的低压制备工具。SUPELCOSIL LC-Si 柱中的未改性硅胶也可分离位置异构体。
法律資訊
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
J J Agren et al.
Lipids, 37(6), 613-619 (2002-07-18)
Normal-phase HPLC resolution of sn-1,2(2,3)- and x-1,3-DAG generated by partial Grignard degradation from natural TAG was carried out with both (R)-(-) and (S)-(+)-1-(1-naphthyl)ethylurethane derivatives. The diastereomeric sn-1,2- and sn-2,3-DAG derivatives were resolved using two Supelcosil LC-Si (5 microm, 25 cm
Mohammed Shahid Ali et al.
Journal of pharmaceutical and biomedical analysis, 43(1), 158-167 (2006-08-05)
A new HILIC method has been developed for the simultaneous determination of pseudoephedrine hydrochloride (PSH), diphenhydramine hydrochloride (DPH) and dextromethorphan hydrobromide (DXH) in cough-cold syrup. Mobile phase consists of methanol:water (containing 6.0 g of ammonium acetate and 10 mL of
R Lovlin et al.
Journal of chromatography. B, Biomedical applications, 679(1-2), 196-198 (1996-04-26)
The stereospecific HPLC assays reported for ketoprofen (KT) mainly utilize indirect approaches. These assays involve the formation of amide diastereomeric derivatives, which are then separated by chromatography. The advantages of indirect methods include versatility, good sensitivity and cost effectiveness; however
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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