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581325-U

Supelco

Ascentis® C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

别名:

Ascentis RP18 HPLC色谱柱

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About This Item

分類程式碼代碼:
41115700
eCl@ss:
32110501
NACRES:
SB.52

产品名称

Ascentis ® C18 HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

材料

stainless steel column

agency

suitable for USP L1 (Similar to Phenomenex Luna C18)

產品線

Ascentis®

特點

endcapped

製造商／商標名

Ascentis®

包裝

1 ea of

標籤範圍

25% Carbon loading

參數

≤70 °C temp. range
400 bar pressure (5801 psi)

技術

HPLC: suitable
LC/MS: suitable

長度 × 內徑

25 cm × 4.6 mm

表面積

450 m2/g

表面覆盖率

3.7 μmol/m2

雜質

<5 ppm metals

基質

fully porous particle
silica gel high purity, spherical

基質活性組

C18 (octadecyl) phase

粒徑

5 μm

孔徑

100 Å

工作pH值範圍

2-8

應用

food and beverages

分離技術

reversed phase

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相关类别

一般說明

Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。

Ascentis C18 是特别稳定可靠的首选 HPLC 色谱柱，即使对于难以分离的化合物也能提供对称的峰形和出色的保留性能。

應用


  • Identification of recombinant human insulin and biosynthetic insulin analogues by multiplexed targeted unlabeled mass spectrometry of proteotypic tryptic peptides.: 本研究利用 Ascentis® C18 HPLC 色谱柱分离蛋白型胰蛋白酶肽，以鉴定胰岛素类似物，重点介绍了其在靶向质谱法进行蛋白质组学分析中的应用(Qasem RJ et al., 2019).。

  • Development and validation of a novel stability-indicating HPLC method for the simultaneous assay of betamethasone-17-valerate, fusidic acid, potassium sorbate, methylparaben and propylparaben in a topical cream preparation.: 本研究开发并验证了一种使用 Ascentis® C18 HPLC 色谱柱同时测定多种化合物的 HPLC 方法，证明了其在药物制剂稳定性测试中的稳健性(Byrne J et al., 2014)。

  • HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.: 本研究采用 Ascentis® C18 HPLC 色谱柱，使用 HPLC-DAD 和 HPLC-ESI-MS/MS 对蜂胶提取物代谢物进行分析，证明了其在复杂天然产物分析中的有效性 (Pellati F et al., 2011)。

  • Practical comparison of 2.7 microm fused-core silica particles and porous sub-2 microm particles for fast separations in pharmaceutical process development.: 本文比较了不同的 HPLC 色谱柱技术，包括 Ascentis® C18，重点介绍了其在制药工艺开发中至关重要的快速分离方面的性能 (Abrahim A et al., 2010)。

特點和優勢

  • 出色的保留性能
  • 优良的峰形对称性
  • 重现性好
  • 与 LC-MS 完全兼容

推薦產品

探索最适于 HPLC 或 LC-MS 分析的 LiChropur 试剂

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

nwg

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
282389-06
282389-05
282389-04
282389-03
282389-02

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Michael Kühl et al.
eLife, 9 (2020-01-22)
Far-red absorbing chlorophylls are constitutively present as chlorophyll (Chl) d in the cyanobacterium Acaryochloris marina, or dynamically expressed by synthesis of Chl f, red-shifted phycobiliproteins and minor amounts of Chl d via far-red light photoacclimation in a range of cyanobacteria
Dipanjan Goswami et al.
Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
Federica Vacondio et al.
Journal of pharmaceutical and biomedical analysis, 46(1), 200-205 (2007-10-26)
A rapid, simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of the imidazole H(3) antagonist ROS203 in rat plasma, using the superior homologue ROS287 as internal standard. Analyses were performed on an Agilent
Federica Pellati et al.
Journal of chromatography. A, 1242, 43-58 (2012-05-09)
In this study, a detailed phytochemical characterization of Echinacea pallida (Nutt.) Nutt. root extracts and dietary supplements was carried out for the first time by developing advanced chromatographic techniques, based on HPLC with diode array (DAD) and electrospray ionization-mass spectrometry
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实验方案

HPLC Analysis of Anthocyanins in Red Wine on Ascentis® C18

-glucoside chloride; Malvidin 3-glucoside; Delphinidin 3-(6-acetylglucoside); Cyanidin 3-(6-acetylglucoside); Petunidin 3-(6-acetylglucoside); Peonidin 3-(6-acetylglucoside); Malvidin 3-(6-acetylglucoside); Malvidin 3-(6-caffeoylglucoside); Petunidin 3-(6-cumarylglucoside); Peonidin 3-(6-cumarylglucoside); Malvidin 3-(6-cumarylglucoside)

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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