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product name
HybridSPE ®-磷脂SPE小柱, 96-well Plate, bed wt. 50 mg, volume 2 mL, pk of 1
品質等級
產品線
HybridSPE®
形狀
solid
成份
bed wt., 50 mg
包裝
pk of 1
技術
solid phase extraction (SPE): suitable
容積
2 mL
基質活性組
zirconia-based phase
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一般說明
HybridSPE-Phospholipid 96 孔板和Ultra固相萃取柱有“孔内”/“柱内”沉淀法可供选择。即先将生物血浆/血清加入孔内或柱内，接着加入酸化乙腈（沉淀剂）。短暂混合/涡旋后，抽真空。96孔板和 Ultra固相萃取柱含有一系列低孔隙率疏水滤膜/筛板，这些填充柱床的滤膜/筛板组件作为深层过滤器，有助于在提取过程中同时去除磷脂和沉淀蛋白。标准 HybridSPE-Phospholipid磷脂固相萃取小柱需要采用“离柱”沉淀法。
應用
- Rapid analysis of 65 pharmaceuticals and 7 personal care products in plasma and whole-body tissue samples of fish using acidic extraction, zirconia-coated silica cleanup, and liquid chromatography-tandem mass spectrometry: This study discusses the advanced application of chromatography and mass spectrometry, which could involve the use of technologies like HybridSPE®-Phospholipid for sample preparation, specifically for rapid analysis in biological matrices (Tanoue et al., 2020).
- Multi LC-MS/MS and LC-HRMS Methods for Determination of 24 Mycotoxins including Major Phase I and II Biomarker Metabolites in Biological Matrices from Pigs and Broiler Chickens: Demonstrates how LC-MS/MS, potentially incorporating HybridSPE®-Phospholipid technology for sample cleanup, facilitates the sensitive and accurate detection of mycotoxins in complex biological samples (Lauwers et al., 2019).
- Evaluation of an Ion Trap Toxtyper Liquid Chromatography With An Ion Trap Mass Spectrometric Instrument (Toxtyper) for Drug of Abuse Screening in Oral Fluid: Explores the utility of specific LC-MS/MS methodologies which could integrate HybridSPE®-Phospholipid for bioanalytical sample cleanup, enhancing drug screening accuracy (Plecko et al., 2018).
- Effective phospholipid removal from plasma samples by solid phase extraction with the use of copper (II) modified silica gel cartridges: This research potentially aligns with the use of HybridSPE®-Phospholipid technologies for phospholipid removal, focusing on enhancing the purity and quality of chromatography samples (Flieger et al., 2017).
- Liquid chromatography mass spectrometry determination of perfluoroalkyl acids in environmental solid extracts after phospholipid removal and on-line turbulent flow chromatography purification: Illustrates a specialized application of chromatography and mass spectrometry where HybridSPE®-Phospholipid could be applied to improve the analysis of environmental samples by efficient phospholipid removal (Mazzoni et al., 2016).
特點和優勢
- 融合了蛋白质沉淀的简单性和固相萃取(SPE)定向除去磷脂的选择性
- 通过完全除去磷脂和沉淀蛋白，减少离子抑制
- 2-3 步通用流程
- 方法开发最少，甚至不需要方法开发
- 有 96 孔和 1 mL 萃取柱规格可供选择
法律資訊
SPE管或板
應用
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
商品
This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.
This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.
This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.
This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.
实验方案
his study demonstrates the analysis of Warfarin in plasma samples utilizing chiral and achiral (reversed-phase) LC-MS and effective sample prep to remove endogenous phospholipids
A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.
Chromatograms
application for HPLC, application for SPEapplication for HPLCapplication for HPLCapplication for SPE, application for LC-MS展开我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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