567228-U

Discovery^® BIO Wide Pore C5 (3 µm) HPLC Columns

L × I.D. 15 cm × 2.1 mm

• 别名: BIO Wide Pore C5 Chromatography Column
• 分類程式碼代碼: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

属性

• product name: Discovery^® BIO Wide Pore C5 高效液相色谱柱, 3 μm particle size, L × I.D. 15 cm × 2.1 mm
• 材料: stainless steel column
• 產品線: Discovery^®
• 特點: endcapped
• 製造商／商標名: Discovery^®
• 包裝: 1 ea of
• 標籤範圍: 3.5% Carbon loading
• 參數: 0-70 °C temperature; 400 bar pressure (5801 psi)
• 技術: HPLC: suitable; LC/MS: suitable
• 長度 × 內徑: 15 cm × 2.1 mm
• 表面積: 100 m²/g
• 表面覆盖率: 4.5 μmol/m²
• 基質: silica particle platform; fully porous particle
• 基質活性組: C5 (pentyl) phase
• 粒徑: 3 μm
• 孔徑: 300 Å
• 工作pH值: 2-8
• 分離技術: reversed phase

说明

一般說明

Discovery BIO Wide Pore C5 设计用于通过 RP-HPLC 对蛋白质和多肽（尤其是疏水性多肽）进行高效可靠分离。C8 或 C18 等长链键合相通常对蛋白质而言其疏水性太强，引起保留时间的过度延长，或者甚至在色谱柱上发生不可逆吸附。因此针对蛋白质的反相 HPLC，通常使用 C3 或 C4 等短链键合相。然而，这些短链键合相通常相对较易于水解，从而缩短色谱柱的使用寿命，而在 pH 较低时这种情况尤为严重。Discovery BIO Wide Pore C5 的洗脱顺序与传统的 C4 相似，但提高了 pH 稳定性，色谱柱的使用寿命更长。由于改进了硅胶和键合相的化学性质，因此通常在 Discovery BIO Wide Pore C5 上获得的分离效率更高。

應用

• Kinetic performance comparison of superficially porous, fully porous and monolithic reversed-phase columns by gradient kinetic plots for the separation of protein biopharmaceuticals.: This study provides a detailed kinetic performance comparison of different types of reversed-phase HPLC columns, including the Discovery^® BIO Wide Pore C5 HPLC Column. The research highlights its efficiency and resolution in separating complex protein biopharmaceuticals, making it a valuable tool for analytical chemists in biopharmaceutical analysis (Jaag et al., 2022).


• RP-LC x RP-LC analysis of a tryptic digest using a combination of totally porous and partially porous stationary phases.: This paper investigates the use of reverse-phase liquid chromatography (RP-LC) with both totally porous and partially porous stationary phases, including the BIO Wide Pore C5 HPLC Column, to analyze tryptic digests. The findings demonstrate its robustness and high resolution in proteomics applications, enabling precise peptide mapping and protein characterization (Mondello et al., 2010).

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法律資訊

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany