ZRB2704

Anti-SPNS2 Antibody, clone 1F4 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 别名: Protein spinster homolog 2, Sphingosine-1-phosphate transporter SPNS2
• 分類程式碼代碼: 12352203
• NACRES: NA.43

属性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: recombinant monoclonal
• 描述: clone 1F4 ; recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 60 kDa
• 純化經由: using Protein A
• 物種活性: human
• 包裝: antibody small pack of 25
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable
• 同型: IgG
• 表位序列: C-terminal half
• Protein ID登錄號: NP_001118230
• UniProt登錄號: Q8IVW8
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 基因資訊: human ... SPNS2(124976)

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1F4 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detect Sphingosine-1-phosphate transporter SPNS2. It targets an epitope within 23 amino acids from the internal region.

免疫原

KLH-conjugated linear peptide corresponding to 23 amino acids from the internal region of human Sphingosine-1-phosphate transporter SPNS2.

應用

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Human heart tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected SPNS2 in Human heart tissue sections.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound SPNS2 peptide with a KD of 9.3 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SPNS2 in ACHN cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Sphingosine-1-phosphate transporter SPNS2 (UniProt: Q8IVW8; also known as Protein spinster homolog 2, SPNS2) is encoded by the SPNS2 gene (Gene ID: 124976) in human. SPNS2, a member of the large family of non-ATP-dependent organic ion transporter, is a multi-pass membrane protein that serves as a lipid transporter and specifically mediates the export of sphingosine-1-phosphate (S1P) and sphinganine-1-phosphate. It regulates levels of S1P and the S1P gradient that exists between the high circulating concentrations of S1P and low tissue levels that control lymphocyte trafficking. It is required for the egress of T-cells from lymph nodes during an immune response by mediating S1P secretion, which generates a gradient that enables activated T-cells to access lymph. It is also essential for the egress of immature B-cells from the bone marrow. SPNS2 also plays a role in innate immunity and SPNS2 knockout animals show reduced IFN- responses to infections due to the inability of natural killer cells to egress from lymph nodes. The deletion of SPNS2 is reported to impair humoral immune responses to immunization, reduced sensitivity to antigen-induced airway inflammation and hypersensitivity and attenuated delayed-type contact hypersensitivity. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chen, H., et al. (2023). Cell 186(12); 2644-2655; Spigiel, S., et al. (2019). J. Lipid Res. 60 (3); 484-489; Fang, V., et al. (2017). Nat. Immunol., 18; 15-25).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重構

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable