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生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1K20, recombinant monoclonal
描述
1K20 Clone
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 98.82 and 101.16 kDa
observed mol wt ~105 kDa
物種活性
mouse, human, rat
物種活性（以同源性預測）
bovine, Xenopus
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
表位序列
C-terminal
Protein ID登錄號
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 1K20 is a ZooMAb® rabbit recombinant monoclonal antibody that detects Glutamate receptors 2 and 3. It targets an epitope within 13 amino acids from the C-terminal, cytoplasmic domain.
免疫原
KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal, cytoplasmic domain of human Glutamate receptor 2.
應用
Quality Control Testing
Evaluated by Western Blotting in Rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GluR-2 in Rat brain tissue lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GluR-2 in human and mouse brain tissue lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GluR-2 in E18 rat cortical cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GluR-2 in human cerebellum tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GluR-2 in Rat brain tissue lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GluR-2 in human and mouse brain tissue lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GluR-2 in E18 rat cortical cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GluR-2 in human cerebellum tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-GluR-2, clone 1K20 ZooMAb®, Cat. No. ZRB1923, is a recombinant Rabbit monoclonal antibody that targets Glutamate receptors 2 and 3 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
標靶描述
Glutamate receptor 2 (UniProt: P42262; also known GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2, Glutamate receptor ionotropic AMPA 2, GluA2) is encoded by the GRIA2 (also known as GLUR2) gene (Gene ID: 2891) in human. Glutamate receptors are synaptic receptors located primarily on the membranes of neuronal cells. These receptors play important roles in neural communication, memory formation, learning, and regulation by mediating glutamate-dependent postsynaptic excitation. Glutamate receptors are divided into two types, ionotropic glutamate receptors (iGluRs) and metabotropic glutamate receptors (mGluRs). iGluRs form ion channel pores upon glutamate binding, while mGluRs indirectly activate ion channels on the plasma membrane via G protein-coupled signaling cascade. Although all iGluRs and mGluRs bind glutamate, they do exhibit different binding activities toward chemical ligands, such as NMDA, kainate, AMPA, L-AP4, ACPD, L-QA, and such distinction is used to divide these receptors further into subtypes. GluR-2 is a multi-pass membrane AMPA type iGluR with two extracellular domains, four transmembrane domains, and three cytoplasmic domains. It is initially synthesized with a signal peptide sequence (aa. 1-24) that is subsequently cleaved off to generate the mature receptor. GluR2 can exist as homotetramer or a heterotetramer that is formed by dimerization of dimers. It is palmitoylated in its resting state and is depalmitoylated upon glutamate stimulation. Palmitoylation at cysteine 610 leads to its Golgi retention and decreased cell surface expression. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
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