ZRB1535

Anti-SLC10A1 Antibody, clone 1B12 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 别名: Sodium/bile acid cotransporter;Cell growth-inhibiting gene 29 protein;Na(+)/bile acid cotransporter;Na(+)/taurocholate transport protein;Sodium/taurocholate cotransporting polypeptide;Solute carrier family 10 member 1
• 分類程式碼代碼: 12352203

属性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1B12, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 38.12 kDa; observed mol wt ~38 kDa
• 純化經由: using Protein A
• 物種活性: human
• 物種活性（以同源性預測）: monkey
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 技術: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal
• Protein ID登錄號: NP_003040
• UniProt登錄號: Q14973
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SLC10A1(6554)

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1B12 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Sodium/bile acid cotransporter (SLC10A1). It targets an epitope within 17 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Sodium/bile acid cotransporter (SLC10A1).

應用

Quality Control Testing

Evaluated by Western Blotting in Human liver tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SLC10A1 in Human liver tissue lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected recombinant Human SLC10A.

Enzyme Immunoassay Analysis (ELISA): Serial dilutions from a representative lot detected recombinant Human SLC10A1 protein.

Immunocytochemistry Analysis: 1:100 dilution from a representative lot detected SLC10A1 in A549 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in Human liver tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SLC10A1 in Human liver tissue lysate.

標靶描述

Sodium/bile acid cotransporter (UniProt: Q14973; also known as Cell growth-inhibiting gene 29 protein, Na(+)/bile acid cotransporter, Na(+)/taurocholate transport protein, Sodium/taurocholate cotransporting polypeptide, Solute carrier family 10 member 1) is encoded by the SLC10A1 (also known as NTCP, GIG29) gene (Gene ID: 6554) in human. SLC10A1 is a multi-pass membrane glycoprotein with eight transmembrane domains. It is expressed exclusively on the basolateral membrane of hepatocytes and serves as a major high-affinity Na+-dependent transporter of conjugated bile salts from plasma into hepatocytes. It plays a key role in the enterohepatic circulation of bile salts necessary for the solubilization and absorption of dietary fat and fat-soluble vitamins. About 75% of the conjugated bile acids and about 50% of unconjugated bile acids are imported via this transporter. The inward Na+ gradient necessary for this process is maintained by a Na+/K+ ATP pump. This process is shown to be extremely efficient, with 75% to 90% first-pass clearance of bile acids from the sinusoidal blood. SLC10A1 exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. Mutations in SLC10A1 gene are reported to cause familial hypercholanemia 2 (FHCA2) that is characterized by persistently higher plasma levels of conjugated bile salts that is apparent from infancy associated with fat malabsorption and impaired absorption of fat-soluble vitamins. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Vaz, FM., et al. (2014). Hepatology. 61(1); 260-267).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable