推荐产品
生物源
rabbit (recombinant)
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
2D18, monoclonal
recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 23.41 kDa
observed mol wt 23 kDa
物種活性
rat, mouse, human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... BAK1(578)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 2D18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Bcl-2 homologous antagonist/killer (BAK). It targets an epitope within 16 amino acids from the N-terminal region.
免疫原
KLH conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Bcl-2 homologous antagonist/killer (BAK).
應用
Anti-Bak, clone 2D18 ZooMAb, Cat. No. ZRB1453, is a recombinant Rabbit monoclonal antibody that specifically detects Bak and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Bak in RAW264.7 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Bak in human urinary bladder tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bak in Jurkat cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Bak in human urinary bladder tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bak in Jurkat cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
Bcl-2 homologous antagonist/killer (UniProt: Q16611; also known as Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK) is encoded by the BAK1 (also known as BAK, BCL2L7, CDN1) gene (Gene ID: 578) in human. BAK is a single-pass membrane protein that plays a role in the mitochondrial apoptosis process. It is homodimeric protein whose homodimerization is zinc-dependent. It can also form heterodimers with BCL2 and BCL2L1. It is expressed in a wide variety of tissues, with highest levels displayed in heart and skeletal muscle. In non-apoptotic cells, BAK constitutively resides in the mitochondrial outer membrane, but upon arrival of cell death signals, it promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM and releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9. BAK contains three BH motifs (BH1: aa 117-136; BH2: aa 169-184; and BH3: aa 74-88) and intact BH3 motif is required by BIK, BID, BAD, and BAX for their pro-apoptotic activity and their interaction with anti-apoptotic members of the Bcl-2 family. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品?
试试我们的产品选型工具.
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门