ZRB06711

Anti-FADD Antibody, clone 11N7, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 别名: FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD
• 分類程式碼代碼: 12352203
• NACRES: NA.43

属性

• 生物源: rabbit (recombinant)
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 11N7, recombinant monoclonal; monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 23.28 kDa; observed mol wt ~26 kDa
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: gene knockdown: suitable using 1:250; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100; western blot: suitable using 1:1,000
• 同型: IgG
• UniProt登錄號: Q13158
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... FADD;MORT1;GIG3(8772)

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 11N7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human FAS-associated death domain protein (FADD).

免疫原

His-tagged full-length recombinant human FAS-associated death domain protein (FADD).

應用

Anti-FADD, clone 11N7, ZooMAb, Cat. No. Cat. No. ZRB06711, is a recombinant Rabbit Monoclonal antibody that detects human FAS-associated death domain and has been tested for use in Immunohistochemistry (Paraffin), RNAi/siRNA/Gene Knockdown, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected FADD in MCF7 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FADD in human kidney tissue sections.

RNAi/siRNA/Gene Knockdown Analysis: A 1:250 dilution from a representative lot detected FADD lysates from Wild type HeLa cells, but not in FADD knockout HeLa cells.

標靶描述

FAS-associated death domain protein (UniProt: Q13158; also known as FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD) is encoded by the FADD (also known as MORT1, GIG3) gene (Gene ID: 8772) in human. FADD is an apoptotic adaptor molecule that bridges death receptor signaling to the caspase cascade. It is expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. FADD contains a death domain at its C-terminus and a death effector domain (DED) at its N-terminal. The death domain is responsible for FADD binding to the activated trimerized Fas receptor. FADD recruits procaspase-8 through its N-terminal DED, which is then activated following the cleavage of pro domain. The death inducing signaling complex (DISC) formed by Fas receptor, FADD, and caspase-8 is essential in triggering apoptotic events in cells. Human FADD is phosphorylated mainly at Ser194. Defects in FADD gene have been linked to recurrent infections, hepatic dysfunction, and cardiovascular malformations. These subjects display high circulating levels of CD4(-) and CD8(-) cells and elevated levels of IL-10. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 2