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WGA2

Sigma-Aldrich

GenomePlex®完整全基因组扩增（WGA）试剂盒

Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue

别名:

Whole genome amplification kit

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About This Item

分類程式碼代碼:
41121800
NACRES:
NA.55

技術

whole genome amplification: suitable

運輸包裝

wet ice

儲存溫度

−20°C

相关类别

一般說明

GenomePlex®全基因组扩增试剂盒采用基于基因组 DNA 随机片段化的专有技术，并将产生的小片段转换为两侧有通用引物结合位点的 PCR 可扩增文库分子。 WGA 采用通用寡核苷酸引物进行文库分子 PCR 扩增。它包括全基因组扩增所需的一切试剂，包括优化的酶 WGA DNA 聚合酶。它可用于多种应用，适用于来自各种来源的纯化基因组 DNA，包括血卡、全血、口腔拭子、土壤、植物和血清。它让研究人员可以生成具有代表性的、约 500 倍的基因组 DNA 扩增。

應用

GenomePlex®完整全基因组扩增（WGA）试剂盒用于DNA扩增.这一试剂盒还适用于下游应用，包括：
  • 芯片分析
  • SNP分析
  • STR分析
  • DNA存档

特點和優勢

  • 以最少的模板获得更高的产量: 在不到 3 小时内将纳克量的基因组 DNA 扩增为微克产量（约 10μ g）
  • 纳克样品可在 –20 °C 下保存以备将来使用
  • 可选择各种DNA来源：全血、口腔拭子、血卡、植物、土壤、&福尔马林固定石蜡包埋的组织（FFPE）
  • 全基因组扩增 （WGA） DNA 聚合酶提高扩增准确度
  • 全基因组无可检出的等位基因偏倚
  • 兼容许多下游应用，例如 TaqMan ®检测、单核苷酸多态性 (SNP) 分析、比较基因组杂交 (CGH) 分析

其他說明

此试剂盒中提供的通用引物序列被认为是专有的。

法律資訊

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护： 5,789,224、5,618,711、6,127,155 和美国境外的权利要求，对应于过期的美国专利号 5,079,352。 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者′内部的研究用途。未通过明示、暗示或禁止反言形式授予购买者任何专利主张下的权利、执行任何专利方法的权利或开展任何形式的商业服务的权利，包括但不限于出于经济利益或其他商业考虑报告购买者′的活动结果。本品仅供研究使用。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • W4502Water, Nuclease-Free Water, for Molecular Biology化学品安全说明书

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCJ9673
SLCK6772
SLCK6770
SLCJ5972
SLCJ4178

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Levi Yant et al.
The Plant cell, 22(7), 2156-2170 (2010-08-03)
The Arabidopsis thaliana transcription factor APETALA2 (AP2) has numerous functions, including roles in seed development, stem cell maintenance, and specification of floral organ identity. To understand the relationship between these different roles, we mapped direct targets of AP2 on a
M Korabecna et al.
Neoplasma, 63(3), 402-410 (2016-03-02)
Tubulocystic renal cell carcinoma (TRCC) represents a rare tumor with incidence lower than 1 % of all renal carcinomas. This study was undertaken to contribute to characterization of molecular signatures associated with TRCC and to compare them with the features of papillary
Tao Chen et al.
Toxicology, 292(2-3), 63-70 (2011-11-15)
Furan, a widely used industrial compound, has been found in a number of heated food items. Furan is carcinogenic to rats and mice, but the mechanism behind its carcinogenic effect is still not well understood. In this study, we tested
Karolina Åberg et al.
European journal of human genetics : EJHG, 20(9), 953-955 (2012-03-02)
DNA from Epstein-Barr virus-transformed lymphocyte cell lines (LCLs) has proven useful for studies of genetic sequence polymorphisms. Whether LCL DNA is suitable for methylation studies is less clear. We conduct a genome-wide methylation investigation using an array set with 45
Zhong Zhao et al.
Nature, 465(7301), 1089-1092 (2010-06-26)
The classic phytohormones cytokinin and auxin play essential roles in the maintenance of stem-cell systems embedded in shoot and root meristems, and exhibit complex functional interactions. Here we show that the activity of both hormones directly converges on the promoters
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Array CGH Analysis of Challenging Samples

In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome, or 0.5 μg, are generally needed per sample to rocess one CGH array.

Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method

In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, somewhat hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome, or 0.5 μg, are generally needed per sample to process one CGH array.

Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis, LOH, aCGH, etc.).

实验方案

Protocol for DNA extraction & WGA Amplification from Soil Samples

Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the GenomePlex® Whole Genome Amplification Protocol is followed

Whole Genome Amplification from Serum or Plasma Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

Saliva DNA Extraction & WGA Amplification Protocol

Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.

Blood Card - Extraction & Amplification WGA Protocol

Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, This protocol provides a simple and convenient method to extract genomic DNA from a blood card. Once the DNA has been extracted, it can then be amplified using the amplification protocol

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相关内容

Animal Tissue DNA-Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.

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