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Merck
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文件

W0138

Sigma-Aldrich

Western Blocker™溶液

for HRP detection systems

别名:

Western blot blocking solution, blocking buffer

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About This Item

分類程式碼代碼:
41105329
NACRES:
NA.25

品質等級

200

儲存期限

≥2 yr

儲存溫度

2-8°C

相关类别

一般說明

在蛋白质通过斑点印迹或蛋白质印迹转移到 PVDF 和硝酸纤维素膜上后，蛋白质印迹封闭液可用于封闭 PVDF 和硝酸纤维素膜。进行封闭是为了减少或消除一抗或二抗的非特异性结合。

應用

蛋白质印迹封闭液已针对使用辣根过氧化物酶（HRP）偶联物的检测系统进行了优化；它也与基于碱性磷酸酶的系统兼容。
蛋白质印迹封闭液已用于封闭蛋白质印迹膜。它也已用于蛋白质印迹中抗体的稀释。

特點和優勢

  • 像许多基于牛奶的封闭液一样，它不会抑制弱 HRP 信号。
  • 既可以用作封闭成分，也可以用作探测成分。
  • 提供即用型，无需制备步骤。

法律資訊

Western Blocker is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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