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Merck

T9028

Sigma-Aldrich

单克隆抗-微管蛋白,酪氨酸 小鼠抗

clone TUB-1A2, ascites fluid

别名:

Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

ascites fluid

抗體產品種類

primary antibodies

無性繁殖

TUB-1A2, monoclonal

包含

15 mM sodium azide

物種活性

human, plant, animal

技術

indirect immunofluorescence: 1:800 using cultured chicken fibroblasts
microarray: suitable
western blot: suitable

同型

IgG3

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

一般說明

单克隆抗络氨酸微管蛋白(小鼠 IgM 同种型)来源于杂交瘤,该杂交瘤由小鼠骨髓瘤细胞与免疫小鼠脾细胞融合产生。微管蛋白为圆柱形丝状结构,几乎存在于所有真核细胞中。微管蛋白是异二聚体,由 α-微管蛋白和 β-微管蛋白组成。 每种亚基的分子量均为55 kDa,具有相当大的同源性。
微管,是细胞内圆柱状丝状结构,主要由微管蛋白组成,存在于所有真核细胞中。单克隆抗酪氨酸抗体可通过间接免疫荧光标记,对酪氨酸 α 微管蛋白进行免疫细胞化学定位。 单克隆抗酪氨酸抗体与酪氨酸微管蛋白发生特异性反应,适用于牛脑细胞、非洲绿猴肾细胞(Vero),犬肾细胞(MDCK)、袋鼠肾细胞(Potoroo,PtK2)、小鼠垂体瘤细胞(AtT-20)、酵母细胞和非洲爪蟾细胞。

特異性

该抗体在免疫印迹测定中与微管蛋白的C-末端酪氨酸反应,可用于培养细胞或组织切片中该表位的定位。

免疫原

含有 α-微管蛋白羧基端氨基酸的肽

應用

单克隆抗酪氨酸微管蛋白用于:
  • 间接免疫荧光标记
  • 免疫印迹技术
  • 免疫细胞化学染色

生化/生理作用

微管蛋白是微管的主要构建单元。微管蛋白的组装过程涉及微管蛋白酪氨酸化。特异性微管蛋白酪氨酸羧肽酶去除末端酪氨酸,产生末端为谷氨酸残基的 α-微管蛋白;而另一种酶通过在羧基末端添加酪氨酸修饰 α-微管蛋白,从而形成酪氨酸增加和损失的潜在循环。

免責聲明

除非我们的产品目录或产品随附的其他公司文件中另有说明,否则我们的产品仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途或任何类型的人类或动物消费或应用。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1


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On some features of embryonic development and metamorphosis of Aurelia aurita (Cnidaria, Scyphozoa)
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Biophysical journal, 93(3), 886-894 (2007-05-15)
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Ceacam6 (carcinoembryonic antigen-related cell adhesion molecule 6 gene) has recently been isolated by differential display followed by RT-PCR and DNA sequence analyses. Ceacam6 is a member of an immunoglobulin superfamily and encodes a protein of 266 amino acid residues possessing
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The TGF-β signaling pathway is a metazoan-specific intercellular signaling pathway known to be important in many developmental and cellular processes in a wide variety of animals. We investigated the complexity and possible functions of this pathway in a member of

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Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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