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Merck

T8159

Sigma-Aldrich

胰蛋白酶磷酸盐肉汤 溶液

sterile-filtered, suitable for cell culture

别名:

TPB solution

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About This Item

分類程式碼代碼:
12352205
NACRES:
NA.75

無菌

sterile-filtered

形狀

solution

濃度

29.5 g/L in deionized water

技術

cell culture | mammalian: suitable

運輸包裝

ambient

儲存溫度

room temp

應用

除了用于需要复杂营养的微生物的生长外,我们还研究了磷锥虫肉汤(TPB)作为培养基制备的补充,该培养基支持BHK-21细胞中疫苗的生产和SF21昆虫细胞在高密度灌注培养搅拌罐生物反应器中的生长。

成分

胰蛋白酶磷酸盐肉汤(TPB)由四种成分组成:胰蛋白酶(20g/L);葡萄糖(2g/L);氯化钠(5g/L)和磷酸二钠(2.5g/L),通常调节至pH 7.3。胰蛋白酶组分是一种蛋白胨(氨基酸和短肽的混合物),由牛奶蛋白酪蛋白的混合酶水解(胰酶)得到。这种水解产物提供基于氨基酸的营养物和生存因子的来源,支持难养微生物如布鲁氏菌、链球菌和奈瑟氏球菌以及真核细胞如昆虫和动物细胞的生长。葡萄糖提供了一种可发酵的碳水化合物,可被需要复杂营养的微生物利用。氯化钠维持渗透和离子平衡,磷酸二钠提供基本缓冲能力。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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