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無菌
sterile-filtered
形狀
solution
濃度
29.5 g/L in deionized water
技術
cell culture | mammalian: suitable
運輸包裝
ambient
儲存溫度
room temp
應用
除了用于需要复杂营养的微生物的生长外,我们还研究了磷锥虫肉汤(TPB)作为培养基制备的补充,该培养基支持BHK-21细胞中疫苗的生产和SF21昆虫细胞在高密度灌注培养搅拌罐生物反应器中的生长。
成分
胰蛋白酶磷酸盐肉汤(TPB)由四种成分组成:胰蛋白酶(20g/L);葡萄糖(2g/L);氯化钠(5g/L)和磷酸二钠(2.5g/L),通常调节至pH 7.3。胰蛋白酶组分是一种蛋白胨(氨基酸和短肽的混合物),由牛奶蛋白酪蛋白的混合酶水解(胰酶)得到。这种水解产物提供基于氨基酸的营养物和生存因子的来源,支持难养微生物如布鲁氏菌、链球菌和奈瑟氏球菌以及真核细胞如昆虫和动物细胞的生长。葡萄糖提供了一种可发酵的碳水化合物,可被需要复杂营养的微生物利用。氯化钠维持渗透和离子平衡,磷酸二钠提供基本缓冲能力。
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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